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Enhancing the Steroid Sulfatase Activity of the Arylsulfatase from Pseudomonas aeruginosa
Australian Natl Univ, Res Sch Chem, Canberra, ACT 2601, Australia.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular biophysics.
Australian Natl Univ, Res Sch Chem, Canberra, ACT 2601, Australia.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Biochemistry. Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Structural Biology.ORCID iD: 0000-0002-3190-1173
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2018 (English)In: ACS Catalysis, ISSN 2155-5435, E-ISSN 2155-5435, Vol. 8, no 9, p. 8902-8914Article in journal (Refereed) Published
Abstract [en]

Steroidal sulfate esters play a central role in many physiological processes. They serve as the reservoir for endogenous sex hormones and form a significant fraction of the steroid metabolite pool. The analysis of steroid sulfates is thus essential in fields such as medical science and sports drug testing. Although the direct detection of steroid sulfates can be readily achieved using liquid chromatography-mass spectrometry, many analytical approaches, including gas chromatography-mass spectrometry, are hampered due to the lack of suitable enzymatic or chemical methods for sulfate ester hydrolysis prior to analysis. Enhanced methods of steroid sulfate hydrolysis would expand analytical possibilities for the study of these widely occurring metabolites. The arylsulfatase from Pseudomonas aeruginosa (PaS) is a purified enzyme capable of hydrolyzing steroid sulfates. However, this enzyme requires improvement to hydrolytic activity and substrate scope in order to be useful in analytical applications. These improvements were sought by applying semirational design to mutate amino acid residues neighboring the enzyme active site. Mutagenesis was implemented on both single and multiple residue sites. Screening by ultra-high performance liquid chromatography-mass spectrometry was performed to test the steroid sulfate hydrolysis activity of these mutant libraries against testosterone sulfate. This approach revealed the steroid sulfate binding pocket and resulted in three mutants that showed an improvement in catalytic efficiency (V-max/K-M) of more than 150 times that of wild-type PaS. The substrate scope of PaS was expanded, and a modest increase in thermostability was observed. Finally, molecular dynamics simulations of enzyme-substrate complexes were used to provide qualitative insight into the structural origin of the observed effects.

Place, publisher, year, edition, pages
AMER CHEMICAL SOC , 2018. Vol. 8, no 9, p. 8902-8914
Keywords [en]
steroid sulfate, sulfate ester, arylsulfatase, steroid sulfatase, Pseudomonas aeruginosa, enzyme promiscuity, enzyme engineering, molecular dynamics
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-366273DOI: 10.1021/acscatal.8b02905ISI: 000444364800122OAI: oai:DiVA.org:uu-366273DiVA, id: diva2:1265715
Funder
Swedish Research Council, 2015-04928Knut and Alice Wallenberg FoundationSwedish National Infrastructure for Computing (SNIC), 2016-34-27Swedish National Infrastructure for Computing (SNIC), 2017-12-11The Wenner-Gren FoundationAvailable from: 2018-11-26 Created: 2018-11-26 Last updated: 2018-11-26Bibliographically approved

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Pabis, AnnaKamerlin, Shina C. Lynn

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