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Characterization of full genome sequences of chicken anemia viruses circulating in Egypt reveals distinct genetic diversity and evidence of recombination
Anim Hlth Res Inst, Natl Lab Vet Qual Control Poultry Prod, Giza 12618, Egypt.
Anim Hlth Res Inst, Natl Lab Vet Qual Control Poultry Prod, Giza 12618, Egypt.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology. Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Infectious Diseases. Anim Hlth Res Inst, Natl Lab Vet Qual Control Poultry Prod, Giza Egypt.ORCID iD: 0000-0001-6876-8903
2018 (English)In: Virus Research, ISSN 0168-1702, E-ISSN 1872-7492, Vol. 251, p. 78-85Article in journal (Refereed) Published
Abstract [en]

Chicken anemia virus (CAV) is one of the commercially important diseases of poultry worldwide. In Egypt, CAV has been reported to be a potential threat to the commercial poultry sectors. Hence, this study was aimed at isolation and full genomic analysis of CAVs circulating in chicken populations in different geographical location in Egypt. A total of 42 samples were collected from broiler chicken flocks in 9 governorates in Egypt from 12 to 42 days of age. The mortality rate observed among chickens was ranging from 3% to 22%. Nineteen out of 42 farms were found positive for the CAV genome by polymerase chain reaction (PCR). Full genome sequencing was conducted for 18 positive samples. Genetic analysis revealed a high similarity of > 99% in 11 viruses with the vaccine strain Del-Ros; while the other seven samples shared close similarity to CAV field strains isolated from China, Taiwan, and Brazil. The data also indicated Q139 and Q144 amino acids substitutions among the VP1 of Egyptian field strains, which are known to be important in virus replication and spread. Phylogenetic analysis of the sequenced viruses (n = 18) based on either the full gene nucleotide sequence or VP1 coding sequence, suggested the circulation of four distinct genotypes in Egypt designated as group A, B, C and D. Moreover, evidence of recombination was detected among four Egyptian CAVs located within group A. The findings of this study succeeded to elucidate the epidemiological and genetic features of CAVs circulating in Egypt, and underscores the important of CAVs surveillance.

Place, publisher, year, edition, pages
ELSEVIER SCIENCE BV , 2018. Vol. 251, p. 78-85
Keywords [en]
Chicken anemia virus, Egypt, Poultry production, Full genome, Recombination event
National Category
Microbiology in the medical area
Identifiers
URN: urn:nbn:se:uu:diva-358160DOI: 10.1016/j.virusres.2018.05.008ISI: 000435047600011PubMedID: 29751020OAI: oai:DiVA.org:uu-358160DiVA, id: diva2:1268003
Available from: 2018-12-04 Created: 2018-12-04 Last updated: 2018-12-04Bibliographically approved

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