uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Interaction of hepatitis C virus NS5A with La protein revealed by T7 phage display
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology. (Anders Bergqvist)
2003 (English)In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, Vol. 309, no 3, 695-701 p.Article in journal (Refereed) Published
Abstract [en]

Although the hepatitis C virus (HCV) genome is synthesized by the virus-encoded RNA-dependent RNA polymerase NS5B, other viral and cellular factors are assumed to be required for template-specific initiation and regulation of RNA-synthesis. The cellular protein La, which normally associates with RNA polymerase III transcripts, also interacts with the 5'- and 3'-untranslated regions of several RNA viruses, including HCV. To investigate whether other viral gene products may be involved in this interaction, we constructed an HCV cDNA expression library in bacteriophage T7 allowing portions of the HCV polyprotein to be displayed on the phage surface. Screening of the phage library against La resulted in selection of clones displaying the N-terminal region of HCV NS5A. Co-precipitation of full-length and truncated forms of recombinant NS5A with La revealed that the N-terminal region of NS5A was both necessary and sufficient for binding to La. Although this region of NS5A is essential for HCV replication, the role of the NS5A-La interaction in the infected cell remains to be established.

Place, publisher, year, edition, pages
2003. Vol. 309, no 3, 695-701 p.
Keyword [en]
Hepatitis C virus, La antigen, NS5A, cDNA library, Phage T7
National Category
Microbiology in the medical area
Identifiers
URN: urn:nbn:se:uu:diva-86668DOI: 10.1016/j.bbrc.2003.08.054PubMedID: 12963047OAI: oai:DiVA.org:uu-86668DiVA: diva2:126850
Available from: 2008-11-27 Created: 2008-11-24 Last updated: 2009-05-26Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Authority records BETA

Bergqvist, Anders

Search in DiVA

By author/editor
Bergqvist, Anders
By organisation
Department of Medical Biochemistry and Microbiology
In the same journal
Biochemical and Biophysical Research Communications - BBRC
Microbiology in the medical area

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 476 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf