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Principles for different modes of multiple-injection CZE
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Medicinal Chemistry, Analytical Pharmaceutical Chemistry.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Medicinal Chemistry, Analytical Pharmaceutical Chemistry.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Medicinal Chemistry, Analytical Pharmaceutical Chemistry.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Medicinal Chemistry, Analytical Pharmaceutical Chemistry.
2008 (English)In: Electrophoresis, ISSN 0173-0835, E-ISSN 1522-2683, Vol. 29, no 19, p. 3952-3958Article in journal (Refereed) Published
Abstract [en]

This paper introduces four different modes of multiple-injection CZE (MICZE). The validity of these MICZE models was evaluated by the experimental data. Prior to the application of MICZE, the electrophoretic conditions are developed in the single-injection mode by adjusting different experimental parameters such as pH, type and concentration of buffer additives and temperature. Based on the migration time difference (tmig) between the analyte and the internal standard or injection marker, one or more MICZE modes can be employed. The injection marker is added to the sample to compensate for injection-volume fluctuations. The inter-plug distance is regulated by applying an electrical field over the capillary for a short period of time between each injection. After the final injection, the separation is completed by electrophoresis for a time period corresponding to that in the single-injection mode

Place, publisher, year, edition, pages
Wiley , 2008. Vol. 29, no 19, p. 3952-3958
Keyword [en]
Moxonidine, Multiple-injection, Oxprenolol, Phenylpropanolamine, Salbutamol
National Category
Medicinal Chemistry
Research subject
Analytical Pharmaceutical Chemistry
Identifiers
URN: urn:nbn:se:uu:diva-86740DOI: 10.1002/elps.200800398ISI: 000261049400002OAI: oai:DiVA.org:uu-86740DiVA: diva2:127160
Note
Conference Information: 7th Asia-Pacific International Symposium on Microscale Separations and Analysis Singapore, SINGAPORE, DEC 17-19, 2007 Available from: 2008-12-01 Created: 2008-12-01 Last updated: 2018-01-13Bibliographically approved
In thesis
1. Separation of Pharmaceuticals by Capillary Electrophoresis using Partial Filling and Multiple-injections
Open this publication in new window or tab >>Separation of Pharmaceuticals by Capillary Electrophoresis using Partial Filling and Multiple-injections
2008 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Different multiple-injection methodologies and the partial filling technique (PFT) have been utilized for separation of pharmaceuticals by capillary elec-trophoresis.

In multiple-injection capillary zone electrophoresis (MICZE), the samples and all standards, used for construction of the calibration curve, are analyzed within a single run. Four different modes of MICZE have been described by means of equations, which were experimentally verified. The developed equations facilitate the transfer from conventional single-injection CZE to one or more of these MICZE-modes, depending on the selectivity between the analyte and the injection marker. The applicability of two of these modes was then demonstrated by quantification of buserelin and salbutamol, re-spectively in commercially available pharmaceutical products. The content of buserelin in an injection solution was determined to 0.94 mg/ml, which only deviated slightly from the declared concentration (1 mg/ml). An alter-native mode of MICZE, offering a higher number of sequential sample injec-tions, was then utilized for single-run determination of salbutamol in 15 tab-lets, with a labelled content of 8 mg. The average content of the tablets was determined to 7.8 mg, with an intra-tablet variation of 3 % or less.

Moreover, UV- and mass-spectrometric detection of enantiomeric amines, resolved by non-aqueous capillary electrophoresis (NACE), was demon-strated. Separation of enantiomeric amines was achieved using the chiral selector (-)-2,3:4,6-di-O-isopropylidene-2-keto-L-gulonic acid, (-)-DIKGA. Introduction of the non-volatile (-)-DIKGA into the mass-spectrometer was avoided by using the PFT, where the capillary is only partially filled with electrolyte containing the chiral selector.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2008. p. 60
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Pharmacy, ISSN 1651-6192 ; 75
Keyword
Capillary Coating, Chiral Separation, Enantiomers, Mass-spectrometric Detection, Method Validation, Multiple-injection Capillary Zone Electrophoresis, Non-aqueous Capillary Electrophoresis, Organic Solvents, Pharmaceutical Analysis, Quantitative Analysis
Identifiers
urn:nbn:se:uu:diva-8841 (URN)978-91-554-7211-5 (ISBN)
Public defence
2008-06-05, B41, BMC, Husargatan 3, Uppsala, 13:15
Opponent
Supervisors
Available from: 2008-05-14 Created: 2008-05-14 Last updated: 2011-01-28Bibliographically approved

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Amini, AhmadLodén, HenrikPettersson, CurtArvidsson, Torbjörn

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