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Ribosomal incorporation of unnatural amino acids: lessons and improvements from fast kinetics studies
Stanford Univ, Sch Med, Dept Struct Biol, Stanford, CA 94305 USA.ORCID iD: 0000-0003-4170-9289
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology.ORCID iD: 0000-0002-5844-6916
2018 (English)In: Current opinion in chemical biology, ISSN 1367-5931, E-ISSN 1879-0402, Vol. 46, p. 180-187Article, review/survey (Refereed) Published
Abstract [en]

Technologies for genetically programming ribosomal incorporation of unnatural amino acids are expanding and have created many exciting applications. However, these applications are generally limited by low efficiencies of the unnatural incorporations. Here we review our current mechanistic understanding of these limitations delineated from in vitro fast kinetics. Rate limitation occurs by different mechanisms, depending on the classes of the unnatural amino acids and the tRNA adaptors. This new understanding has led to several ways of improving the incorporation efficiencies, as well as challenges of dogma on rate-limiting steps in protein synthesis in natural cells.

Place, publisher, year, edition, pages
Elsevier, 2018. Vol. 46, p. 180-187
National Category
Biochemistry and Molecular Biology Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
URN: urn:nbn:se:uu:diva-369950DOI: 10.1016/j.cbpa.2018.07.009ISI: 000449131300025PubMedID: 30125734OAI: oai:DiVA.org:uu-369950DiVA, id: diva2:1272044
Funder
Knut and Alice Wallenberg FoundationSwedish Research Council, 2016-1Swedish Research Council, 2017-04148Available from: 2018-12-18 Created: 2018-12-18 Last updated: 2018-12-18Bibliographically approved

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Forster, Anthony C.

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