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Ligand-Capped Ultrapure Metal Nanoparticle Sensors for the Detection of Cutaneous Leishmaniasis Disease in Exhaled Breath
MINOS-EMaS, Department of Electronics, Electrical and Automatic Engineering, Rovira i Virgili University, Tarragona 43007, Spain.
Institut Pasteur de Tunis, LR11IPT03, Laboratory of Epidemiology and Veterinary Microbiology (LEMV), University Tunis El Manar, Tunis-Belvédère 1002, Tunisia.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Solid State Physics. Molecular Fingerprint AB Sweden, Uppsala 75655, Sweden.ORCID iD: 0000-0003-0296-5247
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Solid State Physics. MINOS-EMaS, Department of Electronics, Electrical and Automatic Engineering, Rovira i Virgili University, Tarragona 43007, Spain.
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2018 (English)In: ACS Sensors, Vol. 3, no 12, p. 2532-2540Article in journal (Refereed) Published
Abstract [en]

Human cutaneous leishmaniasis, although designated as one of the most neglected tropical diseases, remains underestimated due to its misdiagnosis. The diagnosis is mainly based on the microscopic detection of amastigote forms, isolation of the parasite, or the detection of LeishmaniaDNA, in addition to its differential clinical characterization; these tools are not always available in routine daily practice, and they are expensive and time-consuming. Here, we present a simple-to-use, noninvasive approach for human cutaneous leishmaniasis diagnosis, which is based on the analysis of volatile organic compounds in exhaled breath with an array of specifically designed chemical gas sensors. The study was realized on a group of n = 28 volunteers diagnosed with human cutaneous leishmaniasis and a group of n = 32 healthy controls, recruited in various sites from Tunisia, an endemic country of the disease. The classification success rate of human cutaneous leishmaniasis patients achieved by our sensors test was 98.2% accuracy, 96.4% sensitivity, and 100% specificity. Remarkably, one of the sensors, based on CuNPs functionalized with 2-mercaptobenzoxazole, yielded 100% accuracy, 100% sensitivity, and 100% specificity for human cutaneous leishmaniasis discrimination. While AuNPs have been the most extensively used in metal nanoparticle–ligand sensing films for breath sensing, our results demonstrate that chemical sensors based on ligand-capped CuNPs also hold great potential for breath volatile organic compounds detection. Additionally, the chemical analysis of the breath samples with gas chromatography coupled to mass spectrometry identified nine putative breath biomarkers for human cutaneous leishmaniasis.

Place, publisher, year, edition, pages
2018. Vol. 3, no 12, p. 2532-2540
Keywords [en]
biomarkers; chemical gas sensors; diagnosis; exhaled breath analysis; human cutaneous leishmaniasis; metal nanoparticles−ligand nanoassemblies
National Category
Medical Laboratory and Measurements Technologies
Research subject
Engineering Science with specialization in Solid State Physics; Chemistry with specialization in Analytical Chemistry; Engineering Science with specialization in Nanotechnology and Functional Materials
Identifiers
URN: urn:nbn:se:uu:diva-370080DOI: 10.1021/acssensors.8b00759ISI: 000454962400009PubMedID: 30403135OAI: oai:DiVA.org:uu-370080DiVA, id: diva2:1272331
Projects
Tropsense
Funder
EU, Horizon 2020, 645758
Note

De tre första författarna delar förstaförfattarskapet.

Available from: 2018-12-18 Created: 2018-12-18 Last updated: 2019-01-25Bibliographically approved

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Österlund, LarsCindemir, UmutIonescu, Radu

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