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The role of E and N-cadherin in the postoperative course of gonadotroph pituitary tumours
Natl Hosp Norway, Sect Specialized Endocrinol, Dept Endocrinol, Oslo Univ Hosp, Pb 4950 Nydalen, N-0424 Oslo, Norway;Univ Oslo, Fac Med, Oslo, Norway;OUS Rikshosp, Res Inst Internal Med IMF, Postboks 4950 Nydalen, N-0424 Oslo, Norway.
Univ Oslo, Fac Med, Oslo, Norway;Oslo Univ Hosp, Dept Med Biochem, N-0424 Oslo, Norway.ORCID iD: 0000-0003-0157-5888
Natl Hosp Norway, Sect Specialized Endocrinol, Dept Endocrinol, Oslo Univ Hosp, Pb 4950 Nydalen, N-0424 Oslo, Norway;Univ Oslo, Fac Med, Oslo, Norway;OUS Rikshosp, Res Inst Internal Med IMF, Postboks 4950 Nydalen, N-0424 Oslo, Norway.
Univ Oslo, Inst Basic Med Sci, Oslo Ctr Biostat & Epidemiol, Dept Biostat, Oslo, Norway.
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2018 (English)In: Endocrine (Basingstoke), ISSN 1355-008X, E-ISSN 1559-0100, Vol. 62, no 2, p. 351-360Article in journal (Refereed) Published
Abstract [en]

Purpose: Gonadotroph tumours are the most abundant of the clinically silent pituitary tumours. There is a lack of reliable prognostic markers predicting their clinical course. Our aim was to determine the level of E-cadherin and N-cadherin in a cohort of clinically silent gonadotroph pituitary tumours, and compare them to the rate of reintervention.

Methods: Tumour tissue from primary surgery was retrospectively investigated and compared with clinical data. Immunohistochemical (N=105) and real time-qPCR (N=85) analyses for the levels of N-cadherin and the extra- and intracellular domains of E-cadherin were performed. The immunoreactive scores (IRS) and mRNA relative quantity were compared to the rate of reintervention.

Results: The tumours presented a high IRS for N-cadherin (Median 12 (IQR 12-12)) and almost no immunoreactivity for the extracellular domain of E-cadherin (Median 0 (IQR 0-0)). The membranous staining for the intracellular domain of E-cadherin varied (Median 6 (IQR 4-6). Reduced membranous expression of the intracellular domain of E-cadherin was associated with nuclear presence of the same domain. Nuclear staining for the intracellular domain of E-cadherin was associated with a lower rate of reintervention (p=0.01).

Conclusion: We found that silent gonadotroph tumours presented high IRS for N-cadherin and low IRS for the extracellular domain of E-cadherin. A substantial proportion of the tumours presented nuclear staining for the intracellular domain of E-cadherin, accompanied by a reduced membranous expression of the intracellular domain of E-cadherin. Absence of nuclear staining for the intracellular domain of E-cadherin served as an independent predictor of reintervention.

Place, publisher, year, edition, pages
2018. Vol. 62, no 2, p. 351-360
Keywords [en]
Pituitary adenomas, PitNET, EMT, Cadherin, Aggressiveness, Gonadotroph pituitary tumours
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-369589DOI: 10.1007/s12020-018-1679-0ISI: 000449306400011PubMedID: 30051197OAI: oai:DiVA.org:uu-369589DiVA, id: diva2:1272575
Available from: 2018-12-19 Created: 2018-12-19 Last updated: 2018-12-19Bibliographically approved

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