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Identification of novel ejaculate proteins in a seed beetle and division of labour across male accessory reproductive glands
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Ecology and Genetics, Animal ecology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Ecology and Genetics, Animal ecology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Ecology and Genetics, Evolutionary Biology.ORCID iD: 0000-0003-1121-6950
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Ecology and Genetics, Animal ecology.ORCID iD: 0000-0002-3501-3376
2019 (English)In: Insect biochemistry and molecular biology, ISSN 0965-1748, Vol. 104, p. 50-57Article in journal (Refereed) Published
Abstract [en]

The male ejaculate contains a multitude of seminal fluid proteins (SFPs), many of which are key reproductive molecules, as well as sperm. However, the identification of SFPs is notoriously difficult and a detailed understanding of this complex phenotype has only been achieved in a few model species. We employed a recently developed proteomic method involving whole-organism stable isotope labelling coupled with proteomic and transcriptomic analyses to characterize ejaculate proteins in the seed beetle Callosobruchus maculatus. We identified 317 proteins that were transferred to females at mating, and a great majority of these showed signals of secretion and were highly male-biased in expression in the abdomen. These male-derived proteins were enriched with proteins involved in general metabolic and catabolic processes but also with proteolytic enzymes and proteins involved in protection against oxidative stress. Thirty-seven proteins showed significant homology with SFPs previously identified in other insects. However, no less than 92 C. maculatus ejaculate proteins were entirely novel, receiving no significant blast hits and lacking homologs in extant data bases, consistent with a rapid and divergent evolution of SFPs. We used 3D micro-tomography in conjunction with proteomic methods to identify 5 distinct pairs of male accessory reproductive glands and to show that certain ejaculate proteins were only recovered in certain male glands. Finally, we provide a tentative list of 231 candidate female-derived reproductive proteins, some of which are likely important in ejaculate processing and/or sperm storage.

Place, publisher, year, edition, pages
Pergamon , 2019. Vol. 104, p. 50-57
Keywords [en]
Reproductive proteins, Stable isotope labelling, Acps, Sperm competition, Seminal fluid
National Category
Biochemistry and Molecular Biology Zoology
Identifiers
URN: urn:nbn:se:uu:diva-373579DOI: 10.1016/j.ibmb.2018.12.002ISI: 000458938500006PubMedID: 30529580OAI: oai:DiVA.org:uu-373579DiVA, id: diva2:1278943
Funder
Swedish Research Council, 621-2014-4523Knut and Alice Wallenberg FoundationEU, European Research Council, GENCON AdG-294333Available from: 2019-01-15 Created: 2019-01-15 Last updated: 2019-03-11Bibliographically approved

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Bayram, Helen L.Sayadi, AhmedImmonen, ElinaArnqvist, Göran

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