uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
A modified dinucleotide for site-specific RNA-labelling by transcription priming and click chemistry
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Polymer Chemistry. Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Im Neuenheimer Feld 364, Heidelberg .
Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Im Neuenheimer Feld 364, Heidelberg .
Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Im Neuenheimer Feld 364, Heidelberg .
2014 (English)In: Chemical Communications, ISSN 1359-7345, E-ISSN 1364-548X, no 11Article in journal (Refereed) Published
Abstract [en]

An improved strategy for RNA labelling using an alkyne-carrying dinucleotide is reported. This involves near-quantitative priming by phage RNA-polymerases followed by conjugation of different labels using click chemistry. Moreover, these transcripts bear a ligation compatible 5′-end, and thus through ligation the terminal label can be transformed to an internal one.

Place, publisher, year, edition, pages
2014. no 11
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-374170DOI: 10.1039/C3CC46132GOAI: oai:DiVA.org:uu-374170DiVA, id: diva2:1280281
Available from: 2019-01-18 Created: 2019-01-18 Last updated: 2019-08-07Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full texthttps://pubs.rsc.org/en/Content/ArticleLanding/2014/CC/C3CC46132G#!divAbstract

Authority records BETA

Samanta, Ayan

Search in DiVA

By author/editor
Samanta, Ayan
By organisation
Polymer Chemistry
In the same journal
Chemical Communications
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 3 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf