uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Functionalization of RNA oligonucleotides
Universität Heidelberg.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Polymer Chemistry. Universität Heidelberg.
Universität Heidelberg.
Universität Heidelberg.
2013 (English)Patent (Other (popular science, discussion, etc.))
Abstract [en]

The inventors of the present invention developed a two-step method for the post-synthetic enzymatic incorporation of chemical modifications or rather functionalization of RNA oligonucleotides for their labelling. In a first step, the RNA oligonuc - leotide is tailed by the incorporation of a convertible azido-modified (N -modified) nucleotide at the 3'-terminus in an enzymatic re00 action with a nucleotidyl transferase, e.g., the enzyme poly(A) polymerase (PAP) and an N3-modified nucleoside triphosphate (N3 - modified NTP). In a second step, the functionalization is accomplished with an azide-reactive group, e.g., via a copper-catalyzed © 1,3-dipolar cycloaddition reaction between the incorporated N 3 -modified nucleotide and a functional group containing a terminal alkyne moiety (copper-catalyzed click reaction), via a strain-promoted [3 + 2] dipolar cycloaddition between the incorporated N -modified nucleotide and a functional group containing a cyclooctyne moiety (strain-promoted click reaction), or via an azide-phosphine conjugation between the incorporated N -modified nucleotide and a functional group containing a phosphine moiety (Staudinger ligation). To achieve internal chemical modifications or rather functionalization of RNA oligonucleotides, an additional step can be in - troduced between the first and the second step, in which a second RNA oligonucleotide is enzymatically attached to the N -modified nucleotide at the 3'-terminus of the first step, thus converting the 3'-terminal N3-modified nucleotide into an internal N3-modified nucleotide that can be further converted in one of the aforementioned reactions.

Place, publisher, year, edition, pages
2013.
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-374376OAI: oai:DiVA.org:uu-374376DiVA, id: diva2:1280838
Patent
DE WO 2013/013820 AlAvailable from: 2019-01-21 Created: 2019-01-21 Last updated: 2019-01-21

Open Access in DiVA

No full text in DiVA

Other links

https://patentimages.storage.googleapis.com/13/88/3e/0792259509739f/WO2013013820A1.pdf

Authority records BETA

Samanta, Ayan

Search in DiVA

By author/editor
Samanta, Ayan
By organisation
Polymer Chemistry
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar

urn-nbn

Altmetric score

urn-nbn
Total: 13 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf