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Protein measurements in venous plasma, earlobe capillary plasma and in plasma stored on filter paper
Univ Vienna, Dept Anthropol, Althanstr 14, A-1090 Vienna, Austria;Univ Vienna, Dept Behav Biol, Althanstr 14, A-1090 Vienna, Austria.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology. Uppsala University, Science for Life Laboratory, SciLifeLab.
Uppsala University, Science for Life Laboratory, SciLifeLab. Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Molecular tools.
Uppsala University, Science for Life Laboratory, SciLifeLab. Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Molecular tools.
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2019 (English)In: Analytical Biochemistry, ISSN 0003-2697, E-ISSN 1096-0309, Vol. 566, p. 146-150Article in journal (Refereed) Published
Abstract [en]

In this study, levels of inflammatory protein biomarkers in venous plasma, plasma derived from capillary blood from the earlobe, and capillary plasma stored as dried plasma spots (DPS) were compared. Samples from 12 male individuals were assessed with a panel of 92 inflammation-related proteins using multiplex proximity extension assay. Correlations between sample types varied greatly between analytes. A high correlation of rho > 0.8 was observed between capillary plasma and DPS for 32 analytes. At this level of correlation, 13 analytes correlated between venous and capillary plasma and 5 analytes in the comparison of venous blood with DPS.

Place, publisher, year, edition, pages
ACADEMIC PRESS INC ELSEVIER SCIENCE , 2019. Vol. 566, p. 146-150
Keywords [en]
Cytokines, Earlobe capillary, Dried plasma spots, Extension assay, Inflammation protein biomarkers
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
URN: urn:nbn:se:uu:diva-376729DOI: 10.1016/j.ab.2018.11.016ISI: 000456354900024PubMedID: 30472219OAI: oai:DiVA.org:uu-376729DiVA, id: diva2:1287189
Funder
EU, FP7, Seventh Framework Programme, 316929Available from: 2019-02-08 Created: 2019-02-08 Last updated: 2019-02-08Bibliographically approved

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de Oliveira, Felipe Marques SouzaShen, QiujinBjörkesten, JohanKamali-Moghaddam, Masood

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de Oliveira, Felipe Marques SouzaShen, QiujinBjörkesten, JohanKamali-Moghaddam, Masood
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Department of Immunology, Genetics and PathologyScience for Life Laboratory, SciLifeLabMolecular tools
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Analytical Biochemistry
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

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