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Protein Kinase R Is Constitutively Expressed in the Human Pancreas
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Clinical Immunology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Clinical Immunology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Clinical Immunology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Clinical Immunology.
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2019 (English)In: Journal of Histochemistry and Cytochemistry, ISSN 0022-1554, E-ISSN 1551-5044, Vol. 67, no 2, p. 99-105Article in journal (Refereed) Published
Abstract [en]

Viral infection of the insulin-producing cells in the pancreas has been proposed in the etiology of type 1 diabetes. Protein kinase R (PKR) is a cytoplasmic protein activated through phosphorylation in response to cellular stress and particularly viral infection. As PKR expression in pancreatic beta-cells has been interpreted as a viral footprint, this cross-sectional study aimed at characterizing the PKR expression in non-diabetic human pancreases. PKR expression was evaluated in pancreas tissue from 16 non-diabetic organ donors, using immunohistochemistry, qPCR, and western blot. Immunohistochemistry and western blot showed readily detectable PKR expression in the pancreatic parenchyma. The qPCR detected PKR mRNA in both endocrine and exocrine samples, with a slightly higher expression in the islets. In conclusion, PKR is constitutively expressed in both endocrine and exocrine parts of the pancreas and its expression should not be interpreted as a viral footprint in pancreatic beta cells.

Place, publisher, year, edition, pages
SAGE PUBLICATIONS LTD , 2019. Vol. 67, no 2, p. 99-105
Keywords [en]
immunohistochemistry, innate immunity, pancreas, PCR, type 1 diabetes, viral footprints, viral sensors
National Category
Endocrinology and Diabetes
Identifiers
URN: urn:nbn:se:uu:diva-377210DOI: 10.1369/0022155418802838ISI: 000457488700002PubMedID: 30265185OAI: oai:DiVA.org:uu-377210DiVA, id: diva2:1291587
Funder
Swedish Child Diabetes FoundationAvailable from: 2019-02-25 Created: 2019-02-25 Last updated: 2019-02-25Bibliographically approved

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Jonsson, AlexanderYngve, ErikKarlsson, MarieIngvast, SofieSkog, OskarKorsgren, Olle

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