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Graphene Based Mechanical Biosensor by Employing Non-covalent Stacking Functionalization
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.ORCID iD: 0000-0001-7591-2969
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.ORCID iD: 0000-0003-1050-8441
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences. Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Solid State Physics.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Polymer Chemistry. Uppsala University, Science for Life Laboratory, SciLifeLab.
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2019 (English)In: Article in journal, News item (Other academic) Submitted
Abstract [en]

Herein we demonstrate a novel methodology to achieve mechanical biosensor by employing the distinguished interaction forces between the atomic force microscope (AFM) probe and sensor surfaces as the response signal. This mechanical biosensor is fabricated by utilizing the non-covalent π-π stacking of pyrene-maltose onto graphene surfaces with Concanavalin A (Con A) as a target protein. The atomic resolution scanning tunneling microscopy (STM) images indicate the successful formation of the self-assembled and densely packed pyrene-maltose layer on the sensor surface, which gives distinct atomic lattice structure as compared to pristine graphene. This mechanical biosensor exhibits detection of Con A with the sensitivity down to nanomolar level. Therefore, this proposed mechanical biosensor has the potential to be employed in a variety of bio-sensing applications.

Place, publisher, year, edition, pages
2019.
National Category
Nano Technology Analytical Chemistry
Identifiers
URN: urn:nbn:se:uu:diva-378559OAI: oai:DiVA.org:uu-378559DiVA, id: diva2:1294198
Available from: 2019-03-06 Created: 2019-03-06 Last updated: 2019-04-24
In thesis
1. Biosensing platforms using graphene based bioreactive nanostructures with various dimensions
Open this publication in new window or tab >>Biosensing platforms using graphene based bioreactive nanostructures with various dimensions
2019 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Nanomaterials have brought new aspects and improvements to the biosensing field due to their unique physical and chemical properties that are not shown in the bulk state. This thesis focuses on concepting, developing and testing of biosensors where nanomaterials including graphene gold nanoparticles (AuNPs) and magnetic nanoparticles (MNPs) constitute the biosensors. The motivation is to improve the properties of biosensors for protein and nucleic acids by using the nanomaterials’ high surface volume ratio, their unique electrical properties, their good stability and biocompatibility.

The synthesis of well controlled hybrid materials was essential to obtain well performing nucleic acids sensors, whereas a protein sensor contained mainly graphene and organic molecules. The nanomechanical measurements were applied on pyrene-maltose functionalized graphene surfaces after incubating them with the protein. When the Concanavalin A was captured by the pyrene-maltose, the adhesion force of biosensor surface increased significantly. This detection principle was employed to quantify the Concanavalin A attachment to the surface sensitively.

In the development of the eletrocatalytic microRNA sensor, AuNPs were packaged into graphene oxide (GO) sheets to form three-dimensional network structures that both guide the electrical current and increase the surface area of the electrodes. Prior to the assembly of these GO-AuNPs hybrid materials, a duplex-specific nuclease-assisted target recycling reaction was employed for opening the surface of the DNA functionalized AuNPs. The electrocatalytical water splitting activity increased with the fraction of the AuNP surface and thus with the activity of the nuclease-assisted target recycling reaction.

Owing to the high shape anisotropy of graphene, a two-dimensional optomagnetic label GO-MNP nanohybrid was investigated for DNA detection. The DNA coils that were generated through rolling circle amplification absorbed on GO-MNP nanohybrid, leading to a hydrodynamic size increase or aggregation of the proposed nanolabels that can be detected by an optomagnetic sensor. An MNP assembly-based microRNA biosensing strategy is also included in the thesis. DNA scaffolds of the MNP assemblies contain DNAzyme substrate and thus can form cleavage catalytic structures in the presence of microRNA, leading to the disintegration of assemblies. The proposed nanomaterials based biosensing platforms show great potential in the clinical and biomedical applications.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2019. p. 55
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 1779
Keywords
Graphene, nanoparticles, nanostructure, biosensors
National Category
Analytical Chemistry Engineering and Technology
Identifiers
urn:nbn:se:uu:diva-378561 (URN)978-91-513-0587-5 (ISBN)
Public defence
2019-04-25, Room 2005, Ångströmlaboratoriet, Lägerhyddsvägen 1, Uppsala, 13:15 (English)
Opponent
Supervisors
Available from: 2019-04-01 Created: 2019-03-06 Last updated: 2019-05-07

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Han, YuanyuanLi, HuJafri, Syed Hassan MujtabaOssipov, Dmitri A.Hilborn, JönsLEIFER, KLAUS

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