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Perfluorononanoic acid (PFNA) alters lipid accumulation in bovine blastocysts after oocyte exposure during in vitro maturation
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Medicinska och farmaceutiska vetenskapsområdet, centrumbildningar mm, Centre for Reproductive Biology in Uppsala (CRU). Swedish Univ Agr Sci SLU, Dept Clin Sci, Box 7054, SE-7054 Uppsala, Sweden.
Swedish Univ Agr Sci SLU, Dept Clin Sci, Box 7054, SE-7054 Uppsala, Sweden.
Swedish Univ Agr Sci SLU, Dept Clin Sci, Box 7054, SE-7054 Uppsala, Sweden;Swedish Museum Nat Hist, POB 50007, SE-10405 Stockholm, Sweden.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Medicinska och farmaceutiska vetenskapsområdet, centrumbildningar mm, Centre for Reproductive Biology in Uppsala (CRU). Swedish Univ Agr Sci, Dept Biomed Sci & Vet Publ Hlth, Box 7036, SE-75007 Uppsala, Sweden.
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2019 (English)In: Reproductive Toxicology, ISSN 0890-6238, E-ISSN 1873-1708, Vol. 84, p. 1-8Article in journal (Refereed) Published
Abstract [en]

Perfluorononanoic acid (PFNA) is one of the perfluoroalkyl acids present in human tissues. In this study, effects on early embryo development after PFNA exposure were investigated using the bovine in vitro production system. Oocytes were exposed to PFNA during maturation in vitro (10 μg mL-1 and 0.1 μg mL-1), and then fertilized and cultured in parallel with control groups. Developmental parameters (cleavage, blastocyst formation) were followed and embryo quality evaluated (stage, grade). Embryos developed after exposure to 0.1 μg mL-1 were stained to distinguish nuclei, active mitochondria and neutral lipids. 10 μg mL-1 of PFNA had a severe negative effect on blastocyst formation (OR: 0.27 p < 0.05), an effect not observed at 0.1 μg mL-1. However, lipid droplet distribution was significantly altered in embryos exposed to 0.1 μg mL-1, suggesting a disturbance of lipid metabolism after exposure to sublethal levels of PFNA during oocyte maturation in vitro.

Place, publisher, year, edition, pages
2019. Vol. 84, p. 1-8
Keywords [en]
Bovine, IVP, PFNA, Lipid droplet, Image analysis, Developmental toxicology, Endocrine disruption
National Category
Obstetrics, Gynecology and Reproductive Medicine Other Biological Topics Pharmacology and Toxicology
Identifiers
URN: urn:nbn:se:uu:diva-381835DOI: 10.1016/j.reprotox.2018.11.005ISI: 000462417700001PubMedID: 30502403OAI: oai:DiVA.org:uu-381835DiVA, id: diva2:1305138
Funder
Swedish Research Council Formas, 942-2015-476Carl Tryggers foundation , CTS 17:413Available from: 2019-04-15 Created: 2019-04-15 Last updated: 2019-04-15Bibliographically approved

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Reproductive Toxicology
Obstetrics, Gynecology and Reproductive MedicineOther Biological TopicsPharmacology and Toxicology

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