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Self-Assembled Magnetic Nanoparticle−Graphene Oxide Nanotag for Optomagnetic Detection of DNA
Tech Univ Denmark, Dept Hlth Technol DTU Hlth Tech, Lyngby, Denmark.ORCID iD: 0000-0002-5249-4415
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.ORCID iD: 0000-0001-7591-2969
Blusense Diagnost, Copenhagen, Denmark.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Solid State Physics.ORCID iD: 0000-0003-0648-3130
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2019 (English)In: Acs Applied Nano Materials, ISSN 2574-0970, Vol. 2, no 3, p. 1683-1690Article in journal (Refereed) Published
Abstract [en]

In this work, a two-dimensional self-assembled magnetic nanoparticle–graphene oxide (MNP-GO) nanocomposite is reported for the detection of DNA. Single-stranded DNA (ssDNA) coils, generated through a rolling circle amplification (RCA) reaction triggered by the hybridization of target oligos and padlock probes, have a strong interaction with MNP-GO nanotags through several mechanisms including π–π stacking, hydrogen bonding, van der Waals, electrostatic, and hydrophobic interactions. This interaction leads to a hydrodynamic size increase or aggregation of MNP-GO nanotags, which can be detected by a simple optomagnetic setup. Due to the high shape anisotropy, MNP-GO nanotags provide stronger optomagnetic signal than individual MNPs. Moreover, the avoidance of DNA probes (i.e., short ssDNA sequences as the biosensing receptor) provides easier material preparation and lower measurement cost. From real-time measurements of interactions between MNP-GO and RCA products amplified from a highly conserved Escherichia coli 16S rDNA sequence, a limit of detection of 2 pM was achieved with a total assay time of 90 min. Although the nonspecific binding force between GO and ssDNA is much weaker than the specific base-pairing force in a DNA duplex, the proposed method provides a detection limit similar to DNA probe-based magnetic biosensors, which can be ascribed to the abundant binding sites between GO and ssDNA. In addition, for target concentrations higher than 100 pM, the MNP-GO nanotags can be applied for a qualitative naked eye detection strategy based on nanotag–ssDNA flocculation.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2019. Vol. 2, no 3, p. 1683-1690
Keywords [en]
Magnetic nanoparticles; graphene oxide; rolling circle amplification; single stranded DNA detection; optomagnetic sensing
National Category
Nano Technology Analytical Chemistry
Research subject
Analytical Chemistry
Identifiers
URN: urn:nbn:se:uu:diva-378551DOI: 10.1021/acsanm.9b00127ISI: 000462554000061OAI: oai:DiVA.org:uu-378551DiVA, id: diva2:1306641
Available from: 2019-03-06 Created: 2019-04-24 Last updated: 2019-04-24Bibliographically approved
In thesis
1. Biosensing platforms using graphene based bioreactive nanostructures with various dimensions
Open this publication in new window or tab >>Biosensing platforms using graphene based bioreactive nanostructures with various dimensions
2019 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Nanomaterials have brought new aspects and improvements to the biosensing field due to their unique physical and chemical properties that are not shown in the bulk state. This thesis focuses on concepting, developing and testing of biosensors where nanomaterials including graphene gold nanoparticles (AuNPs) and magnetic nanoparticles (MNPs) constitute the biosensors. The motivation is to improve the properties of biosensors for protein and nucleic acids by using the nanomaterials’ high surface volume ratio, their unique electrical properties, their good stability and biocompatibility.

The synthesis of well controlled hybrid materials was essential to obtain well performing nucleic acids sensors, whereas a protein sensor contained mainly graphene and organic molecules. The nanomechanical measurements were applied on pyrene-maltose functionalized graphene surfaces after incubating them with the protein. When the Concanavalin A was captured by the pyrene-maltose, the adhesion force of biosensor surface increased significantly. This detection principle was employed to quantify the Concanavalin A attachment to the surface sensitively.

In the development of the eletrocatalytic microRNA sensor, AuNPs were packaged into graphene oxide (GO) sheets to form three-dimensional network structures that both guide the electrical current and increase the surface area of the electrodes. Prior to the assembly of these GO-AuNPs hybrid materials, a duplex-specific nuclease-assisted target recycling reaction was employed for opening the surface of the DNA functionalized AuNPs. The electrocatalytical water splitting activity increased with the fraction of the AuNP surface and thus with the activity of the nuclease-assisted target recycling reaction.

Owing to the high shape anisotropy of graphene, a two-dimensional optomagnetic label GO-MNP nanohybrid was investigated for DNA detection. The DNA coils that were generated through rolling circle amplification absorbed on GO-MNP nanohybrid, leading to a hydrodynamic size increase or aggregation of the proposed nanolabels that can be detected by an optomagnetic sensor. An MNP assembly-based microRNA biosensing strategy is also included in the thesis. DNA scaffolds of the MNP assemblies contain DNAzyme substrate and thus can form cleavage catalytic structures in the presence of microRNA, leading to the disintegration of assemblies. The proposed nanomaterials based biosensing platforms show great potential in the clinical and biomedical applications.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2019. p. 55
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 1779
Keywords
Graphene, nanoparticles, nanostructure, biosensors
National Category
Analytical Chemistry Engineering and Technology
Identifiers
urn:nbn:se:uu:diva-378561 (URN)978-91-513-0587-5 (ISBN)
Public defence
2019-04-25, Room 2005, Ångströmlaboratoriet, Lägerhyddsvägen 1, Uppsala, 13:15 (English)
Opponent
Supervisors
Available from: 2019-04-01 Created: 2019-03-06 Last updated: 2019-05-07

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Tian, BoHan, YuanyuanStrömberg, MattiasLeifer, Klaus

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