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Inertial focusing with sub-micron resolution for separation of bacteria
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.ORCID iD: 0000-0002-2376-3559
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.ORCID iD: 0000-0003-2744-1634
2019 (English)In: Lab on a Chip, ISSN 1473-0197, E-ISSN 1473-0189, Vol. 19, no 7, p. 1257-1266Article in journal (Refereed) Published
Abstract [en]

In this paper, we study inertial focusing in curved channels and demonstrate the alignment of particles with diameters between 0.5 and 2.0 m, a range of biological relevance since it comprises a multitude of bacteria and organelles of eukaryotic cells. The devices offer very sensitive control over the equilibrium positions and allow two modes of operation. In the first, particles having a large variation in size are focused and concentrated together. In the second, the distribution spreads in a range of sizes achieving separation with sub-micron resolution. These systems were validated with three bacteria species (Escherichia coli, Salmonella typhimurium and Klebsiella pneumoniae) showing good alignment while maintaining the viability in all cases. The experiments also revealed that the particles follow a helicoidal trajectory to reach the equilibrium positions, similar to the fluid streamlines simulated in COMSOL, implying that these positions occupy different heights in the cross section. When the equilibrium positions move to the inner wall as the flow rate increases, they are at a similar distance from the centre than in straight channels (approximate to 0.6R), but when the equilibrium positions move to the outer wall as the flow rate increases, they are closer to the centre and the particles pass close to the inner wall to elevate their position before reaching them. These observations were used along with COMSOL simulations to explain the mechanism behind the local force balance and the migration of particles, which we believe contributes to further understanding of the phenomenon. Hopefully, this will make designing more intuitive and reduce the high pressure demands, enabling manipulation of particles much smaller than a micrometer.

Place, publisher, year, edition, pages
ROYAL SOC CHEMISTRY , 2019. Vol. 19, no 7, p. 1257-1266
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-382383DOI: 10.1039/c9lc00080aISI: 000462723900009PubMedID: 30821308OAI: oai:DiVA.org:uu-382383DiVA, id: diva2:1307023
Funder
EU, Horizon 2020, 644669Available from: 2019-04-25 Created: 2019-04-25 Last updated: 2019-04-25Bibliographically approved

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Cruz, JavierGraells, TiscarHjort, Klas

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Microsystems TechnologyDepartment of Medical Biochemistry and Microbiology
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