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The Low Molecular Mass Photosystem II Protein PsbTn Is Important for Light Acclimation
Univ Umea, Dept Chem, SE-90187 Umea, Sweden;Sichuan Agr Univ, Coll Life Sci, Yaan 625014, Peoples R China.
Sichuan Agr Univ, Coll Resources Sci & Technol, Chengdu 611130, Sichuan, Peoples R China.
Univ Umea, Dept Chem, SE-90187 Umea, Sweden.
Ludwig Maximilians Univ Munchen, Dept Biol 1, Plant Sci, D-82152 Munich, Planegg Martins, Germany.
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2019 (English)In: Plant Physiology, ISSN 0032-0889, E-ISSN 1532-2548, Vol. 179, no 4, p. 1739-1753Article in journal (Refereed) Published
Abstract [en]

Photosystem II (PSII) is a supramolecular complex containing over 30 protein subunits and a large set of cofactors, including various pigments and quinones as well as Mn, Ca, Cl, and Fe ions. Eukaryotic PSII complexes contain many subunits not found in their bacterial counterparts, including the proteins PsbP (PSII), PsbQ, PsbS, and PsbW, as well as the highly homologous, low-molecularmass subunits PsbTn1 and PsbTn2 whose function is currently unknown. To determine the function of PsbTn1 and PsbTn2, we generated single and double psbTn1 and psbTn2 knockout mutants in Arabidopsis (Arabidopsis thaliana). Cross linking and reciprocal coimmunoprecipitation experiments revealed that PsbTn is a lumenal PSII protein situated next to the cytochrome b(559 )subunit PsbE. The removal of the PsbTn proteins decreased the oxygen evolution rate and PSII core phosphorylation level but increased the susceptibility of PSII to photoinhibition and the production of reactive oxygen species. The assembly and stability of PSII were unaffected, indicating that the deficiencies of the psbTn1 psbTn2 double mutants are due to structural changes. Double mutants exhibited a higher rate of nonphotochemical quenching of excited states than the wild type and single mutants, as well as slower state transition kinetics and a lower quantum yield of PSII when grown in the field. Based on these results, we propose that the main function of the PsbTn proteins is to enable PSII to acclimate to light shifts or intense illumination.

Place, publisher, year, edition, pages
AMER SOC PLANT BIOLOGISTS , 2019. Vol. 179, no 4, p. 1739-1753
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Biochemistry and Molecular Biology
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URN: urn:nbn:se:uu:diva-382510DOI: 10.1104/pp.18.01251ISI: 000462993100045PubMedID: 30538167OAI: oai:DiVA.org:uu-382510DiVA, id: diva2:1308054
Funder
Carl Tryggers foundation German Research Foundation (DFG), ME1794/7German Research Foundation (DFG), TRR 175 TP A03German Research Foundation (DFG), TRR175 TP B06Available from: 2019-04-30 Created: 2019-04-30 Last updated: 2019-04-30Bibliographically approved

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Mamedov, Fikret

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