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Luminescent CeO2:Eu3+ nanocrystals for robust in situ H2O2 real-time detection in bacterial cell cultures
Karolinska Inst, Dept Microbiol Tumor & Cell Biol, SE-17177 Stockholm, Sweden;Imperial Coll London, Dept Comp, London, England.
Karolinska Inst, Dept Microbiol Tumor & Cell Biol, SE-17177 Stockholm, Sweden.ORCID iD: 0000-0002-8922-3774
Karolinska Inst, Dept Microbiol Tumor & Cell Biol, SE-17177 Stockholm, Sweden.
Karolinska Inst, Dept Microbiol Tumor & Cell Biol, SE-17177 Stockholm, Sweden;Karolinska Univ Hosp, Dept Clin Microbiol, SE-17176 Stockholm, Sweden.ORCID iD: 0000-0001-5249-9120
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2019 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 132, p. 286-293Article in journal (Refereed) Published
Abstract [en]

Hydrogen peroxide (H2O2) quantification in biomedicine is valuable as inflammation biomarker but also in assays employing enzymes that generate or consume H2O2 linked to a specific biomarker. Optical H2O2 detection is typically performed through peroxidase-coupled reactions utilizing organic dyes that suffer, however, from poor stability/reproducibility and also cannot be employed in situ in dynamic complex cell cultures to monitor H2O2 levels in real-time. Here, we utilize enzyme-mimetic CeO2 nanocrystals that are sensitive to H2O2 and study the effect of H2O2 presence on their electronic and luminescent properties. We produce and dope with Eu3+ these particles in a single-step by flame synthesis and directly deposit them on Si and glass substrates to fabricate nanoparticle layers to monitor in real-time and in situ the H2O2 concentrations generated by Streptococcus pneumoniae clinical isolates. Furthermore, the small CeO2:Eu3+ nanocrystals are combined in a single-step with larger, non-responsive Y2O3:Tb3+ nanoparticles during their double-nozzle flame synthesis to engineer hybrid luminescent nanoaggregates as ratiometric robust biosensors. We demonstrate the functionality of these biosensors by monitoring their response in the presence of a broad range of H2O2 concentrations in vitro from S. pneumoniae, highlighting their potential for facile real-time H2O2 detection in vitro in cell cultures.

Place, publisher, year, edition, pages
ELSEVIER ADVANCED TECHNOLOGY , 2019. Vol. 132, p. 286-293
Keywords [en]
Nanozymes, Rare-earth doped nanoparticles, Hydrogen peroxide, Flame nanoparticle synthesis
National Category
Materials Chemistry
Identifiers
URN: urn:nbn:se:uu:diva-382822DOI: 10.1016/j.bios.2019.03.012ISI: 000464296300033PubMedID: 30884315OAI: oai:DiVA.org:uu-382822DiVA, id: diva2:1313703
Funder
EU, European Research Council, 758705Swedish Research Council, 2016-03471Åke Wiberg FoundationAvailable from: 2019-05-06 Created: 2019-05-06 Last updated: 2019-05-06Bibliographically approved

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Xie, LingLeifer, Klaus

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