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Paradoxical effects of the epigenetic modifiers 5-aza-deoxycytidine and suberoylanilide hydroxamic acid on adipogenesis
Univ Sharjah, Sharjah Inst Med & Hlth Res, Sharjah 27272, U Arab Emirates;Suez Canal Univ, Fac Med, Ismailia 41522, Egypt.
Univ Sharjah, Sharjah Inst Med & Hlth Res, Sharjah 27272, U Arab Emirates.
Univ Sharjah, Res Inst Sci & Engn, Sharjah 27272, U Arab Emirates.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmacy. Univ Sharjah, Sharjah Inst Med & Hlth Res, Sharjah 27272, U Arab Emirates.
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2019 (English)In: Differentiation, ISSN 0301-4681, E-ISSN 1432-0436, Vol. 106, p. 1-8Article in journal (Refereed) Published
Abstract [en]

Adipogenesis is an important biological process that is linked to obesity and metabolic disorders. On the other hand, fat regeneration is crucial as a restorative approach following mastectomy or severe burn injury. Furthermore, optimizing an in-vitro model of adipogenesis, which would help in understanding the possible effects and/or side effects of fat-soluble drugs and anti-obesity remedies, in addition to the developmental studies. Epigenetic is an important factor that is involved in cellular differentiation and commitment. This study aimed at investigating the effect of DNA methylation and histone deactylases inhibitors, 5-Aza-deoxycytidine (5Aza-dC) and Suberoylanilide hydroxamic acid (SAHA), on the adipogenic differentiation process. The two modifiers were applied according to our previously published protocol, followed by three cycles of a classical, two-step adipogenesis protocol. The cells pretreated with SAHA showed enhanced expression of the many adipogenic genes, including peroxisome proliferator-activated receptor-gamma as well as the accumulation of intracytoplasmic fat as shown by oil red and Nile red staining and the secretion of adipokines, such as MCP-1 and IP-10. On contrary, 5-Aza-dC inhibited all these markers. In conclusion, adding the reported step with SAHA to the differentiation protocols could have an impact on the progress of the in-vitro fat regenerative approach. The possible role of 5-Aza-dC in the inhibition of adipogenesis can be of clinical interest and will need further characterization in the future.

Place, publisher, year, edition, pages
Elsevier, 2019. Vol. 106, p. 1-8
Keywords [en]
5-Aza-deoxycytidine, Adipogenesis, Epigenetic modifier, Stem cells, Suberoylanilide hydroxamic acid
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-382474DOI: 10.1016/j.diff.2019.02.003ISI: 000463345400001PubMedID: 30818187OAI: oai:DiVA.org:uu-382474DiVA, id: diva2:1315095
Available from: 2019-05-10 Created: 2019-05-10 Last updated: 2019-05-10Bibliographically approved

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