uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Identification of rfk-1, a Meiotic Driver Undergoing RNA Editing in Neurospora
Illinois State Univ, Sch Biol Sci, 346 Sci Lab Bldg, Normal, IL 61790 USA.
Illinois State Univ, Sch Biol Sci, 346 Sci Lab Bldg, Normal, IL 61790 USA.
Illinois State Univ, Sch Biol Sci, 346 Sci Lab Bldg, Normal, IL 61790 USA.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Organismal Biology, Systematic Biology.
Show others and affiliations
2019 (English)In: Genetics, ISSN 0016-6731, E-ISSN 1943-2631, Vol. 212, no 1, p. 93-110Article in journal (Refereed) Published
Abstract [en]

Sk-2 is a meiotic drive element that was discovered in wild populations of Neurospora fungi over 40 years ago. While early studies quickly determined that Sk-2 transmits itself through sexual reproduction in a biased manner via spore killing, the genetic factors responsible for this phenomenon have remained mostly unknown. Here, we identify and characterize rfk-1, a gene required for Sk-2-based spore killing. The rfk-1 gene contains four exons, three introns, and two stop codons, the first of which undergoes RNA editing to a tryptophan codon during sexual development. Translation of an unedited rfk-1 transcript in vegetative tissue is expected to produce a 102-amino acid protein, whereas translation of an edited rfk-1 transcript in sexual tissue is expected to produce a protein with 130 amino acids. These findings indicate that unedited and edited rfk-1 transcripts exist and that these transcripts could have different roles with respect to the mechanism of meiotic drive by spore killing. Regardless of RNA editing, spore killing only succeeds if rfk-1 transcripts avoid silencing caused by a genome defense process called meiotic silencing by unpaired DNA (MSUD). We show that rfk-1's MSUD avoidance mechanism is linked to the genomic landscape surrounding the rfk-1 gene, which is located near the Sk-2 border on the right arm of chromosome III. In addition to demonstrating that the location of rfk-1 is critical to spore-killing success, our results add to accumulating evidence that MSUD helps protect Neurospora genomes from complex meiotic drive elements.

Place, publisher, year, edition, pages
GENETICS SOCIETY AMERICA , 2019. Vol. 212, no 1, p. 93-110
Keywords [en]
Genetics of Sex, meiotic drive, meiotic silencing, RNA editing, spore killing, transmission ratio distortion
National Category
Biochemistry and Molecular Biology Genetics
Identifiers
URN: urn:nbn:se:uu:diva-383846DOI: 10.1534/genetics.119.302122ISI: 000466802000007PubMedID: 30918007OAI: oai:DiVA.org:uu-383846DiVA, id: diva2:1318015
Funder
EU, Horizon 2020, 648143Swedish Research CouncilAvailable from: 2019-05-24 Created: 2019-05-24 Last updated: 2019-05-24Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMed

Authority records BETA

Svedberg, JesperJohannesson, Hanna

Search in DiVA

By author/editor
Svedberg, JesperJohannesson, Hanna
By organisation
Systematic Biology
In the same journal
Genetics
Biochemistry and Molecular BiologyGenetics

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 5 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf