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Interaction of Laponite with Membrane Components - Consequences for Bacterial Aggregation and Infection Confinement
Univ Copenhagen, Dept Pharm, Wound Healing Ctr, Bispebjerg Hosp, DK-2100 Copenhagen, Denmark.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmacy.ORCID iD: 0000-0001-5236-9107
Univ Copenhagen, Dept Pharm, Wound Healing Ctr, Bispebjerg Hosp, DK-2100 Copenhagen, Denmark.
Univ Copenhagen, Dept Pharm, Wound Healing Ctr, Bispebjerg Hosp, DK-2100 Copenhagen, Denmark.
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2019 (English)In: ACS Applied Materials and Interfaces, ISSN 1944-8244, E-ISSN 1944-8252, Vol. 11, no 17, p. 15389-15400Article in journal (Refereed) Published
Abstract [en]

The antimicrobial effects of Laponite nano particles with or without loading of the antimicrobial peptide LL-37 was investigated along with their membrane interactions. The study combines data from ellipsometry, circular dichroism, fluorescence spectroscopy, particle size/zeta potential measurements, and confocal microscopy. As a result of the net negative charge of Laponite, loading of net positively charged LL-37 increases with increasing pH. The peptide was found to bind primarily to the outer surface of the Laponite nanoparticles in a predominantly helical conformation, leading to charge reversal. Despite their net positive charge, peptide-loaded Laponite nanoparticles did not kill Gram-negative Escherichia coli bacteria or disrupt anionic model liposomes. They did however cause bacteria flocculation, originating from the interaction of Laponite and bacterial lipopolysaccharide (LPS). Free LL-37, in contrast, is potently antimicrobial through membrane disruption but does not induce bacterial aggregation in the concentration range investigated. Through LL-37 loading of Laponite nanoparticles, the combined effects of bacterial flocculation and membrane lysis are observed. However, bacteria aggregation seems to be limited to Gram-negative bacteria as Laponite did not cause flocculation of Gram-positive Bacillus subtilis bacteria nor did it bind to lipoteichoic acid from bacterial envelopes. Taken together, the present investigation reports several novel phenomena by demonstrating that nanoparticle charge does not invariably control membrane destabilization and by identifying the ability of anionic Laponite nanoparticles to effectively flocculate Gram-negative bacteria through LPS binding. As demonstrated in cell experiments, such aggregation results in diminished LPS-induced cell activation, thus outlining a promising approach for confinement of infection and inflammation caused by such pathogens.

Place, publisher, year, edition, pages
AMER CHEMICAL SOC , 2019. Vol. 11, no 17, p. 15389-15400
Keywords [en]
antimicrobial, bacteria flocculation, infection confinement, Laponite, membrane
National Category
Physical Chemistry
Identifiers
URN: urn:nbn:se:uu:diva-383840DOI: 10.1021/acsami.9b03527ISI: 000466988800022PubMedID: 30951282OAI: oai:DiVA.org:uu-383840DiVA, id: diva2:1318136
Funder
Swedish Research Council, 2016-05157Swedish Research Council, 2017-02341Available from: 2019-05-27 Created: 2019-05-27 Last updated: 2019-05-27Bibliographically approved

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Nyström, LinaMalmsten, Martin

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