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Autogenic analyte translocation in nanopores
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Solid State Electronics.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Solid State Electronics.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Solid State Electronics.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Solid State Electronics.ORCID iD: 0000-0003-4317-9701
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2019 (English)In: Nano Energy, ISSN 2211-2855, E-ISSN 2211-3282, Vol. 60, p. 503-509Article in journal (Refereed) Published
Abstract [en]

Nanopores have been widely studied for power generation and single-molecule detection. Although the power level generated by a single nanopore based on electrolyte concentration gradient is too low to be practically useful, such a power level is found sufficient to drive analyte translocation in nanopores. Here, we explore the simultaneous action of a solid-state nanopore as a nanopower generator and a nanoscale biosensor by exploiting the extremely small power generated to drive the analyte translocation in the same nanopore device. This autogenic analyte translocation is demonstrated using protein and DNA for their distinct shape, size and charge. The simple device structure allows for easy implementation of either electrical or optical readout. The obtained nanopore translocation is characterized by typical behaviors expected for an ordinary nanopore sensor powered by an external source. Extensive numerical simulation confirms the power generation and power level generated. It also reveals the fundamentals of autogenic translocation. As it requires no external power source, the sensing can be conducted with simple readout electronics and may allow for integration of high-density nanopores. Our approach demonstrated in this work may pave the way to practical high-throughput single-molecule nanopore sensing powered by the distributed energy harvested by the nanopores themselves.

Place, publisher, year, edition, pages
Elsevier, 2019. Vol. 60, p. 503-509
National Category
Nano Technology
Identifiers
URN: urn:nbn:se:uu:diva-384648DOI: 10.1016/j.nanoen.2019.03.092ISI: 000467774100056OAI: oai:DiVA.org:uu-384648DiVA, id: diva2:1321194
Funder
Swedish Research Council, 621-2014-6300Stiftelsen Olle Engkvist Byggmästare, 2016/39Available from: 2019-06-07 Created: 2019-06-07 Last updated: 2019-06-19Bibliographically approved
In thesis
1. Solid-State Nanopores for Sensing: From Theory to Applications
Open this publication in new window or tab >>Solid-State Nanopores for Sensing: From Theory to Applications
2019 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Nanopore based sensing technology has been widely studied for a broad range of applications including DNA sequencing, protein profiling, metabolite molecules, and ions detection. The nanopore technology offers an unprecedented technological solution to meeting the demands of precision medicine on rapid, in-field, and low-cost biomolecule analysis. In general, nanopores are categorized in two families: solid-state nanopore (SSNP) and biological nanopore. The former is formed in a solid-state membrane made of SiNx, SiO2, silicon, graphene, MoS2, etc., while the latter represents natural protein ion-channels in cell membranes. Compared to biological pores, SSNPs are mechanically robust and their fabrication is compatible with traditional semiconductor processes, which may pave the way to their large-scale fabrication and high-density integration with standard control electronics. However, challenges remain for SSNPs, including poor stability, low repeatability, and relatively high background noise level. This thesis explores SSNPs from basic physical mechanisms to versatile applications, by entailing a balance between theory and experiment.

The thesis starts with theoretical models of nanopores. First, resistance of the open pore state is studied based on the distribution of electric field. An important concept, effective transport length, is introduced to quantify the extent of the high field region. Based on this conductance model, the nanopores size of various geometrical shapes can be extracted from a simple resistance measurement. Second, the physical causality of ionic current rectification of geometrically asymmetrical nanopores is unveiled. Third, the origin of low-frequency noise is identified. The contribution of each noise component at different conditions is compared. Forth, a simple nano-disk model is used to describe the blockage of ionic current caused by DNA translocation. The signal and noise properties are analyzed at system level.

Then, nanopore sensing experiments are implemented on cylinder SiNx nanopores and truncated-pyramid silicon nanopores (TPP). Prior to a systematic study, a low noise electrical characterization platform for nanopore devices is established. Signal acquisition guidelines and data processing flow are standardized. The effects of electroosmotic vortex in TPP on protein translocation dynamics are excavated. The autogenic translocation of DNA and proteins driven by the pW-level power generated by an electrolyte concentration gradient is demonstrated. Furthermore, by extending to a multiple pore system, the group translocation behavior of nanoparticles is studied. Various application scenarios, different analyte categories and divergent device structures accompanying with flexible configurations clearly point to the tremendous potential of SSNPs as a versatile sensor.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2019. p. 108
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 1825
Keywords
solid-state nanopore, ionic current, current blockage, effective transport length, noise, surface charge, translocation, biomolecule, electroosmotic flow, vortex, autogenic translocation, multiple nanopores
National Category
Nano Technology Electrical Engineering, Electronic Engineering, Information Engineering
Research subject
Engineering Science with specialization in Electronics
Identifiers
urn:nbn:se:uu:diva-384667 (URN)978-91-513-0689-6 (ISBN)
Public defence
2019-09-06, Polhemsalen, Ångströmlaboratoriet, Lägerhyddsvägen 1, Uppsala, 13:15 (English)
Opponent
Supervisors
Available from: 2019-08-12 Created: 2019-06-19 Last updated: 2019-08-23

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Wen, ChenyuLi, ShiyuZeng, ShuangshuangZhang, ZhenZhang, Shi-Li

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