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Effect of LPS on extracellular Dscam regulation in P. leniusculus hemocytes
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Organismal Biology, Comparative Physiology. Uppsala University, Disciplinary Domain of Science and Technology, Biology, Biology Education Centre.
2019 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Hemocytes are an important part of a crayfish’s immune system in helping tackling both virus and bacterial infections. Dscam is a protein that can be found in hemocytes, as well as many other tissues like the brain. In the brain, Dscam is thought to be important in the establishment of neuronal connections. Previous studies have found that the neurons in the crayfish brain do not replenish themselves, but instead are replenished by hemocytes that enter through a vascular cavity that pass through the neurogenic niche. There might be a specific type of hemocyte that is drawn to the niche and because of the link between Dscam and establishment of neuronal connections, Dscam have been chosen as a potential factor for this attraction. Dscam could be upregulated at many places along the way from the HPT to the brain. In this study, antibodies have been used to view BrdU and Dscam presence in hemocytes from crayfish P. leniusculus to find out where Dscam is upregulated and in what cells they are located. It was found that Dscam is not present on newly synthesized cells but rather on more differentiated cells, suggesting that Dscam is upregulated in older HPT cells or in circulation. It was found that LPS injections are an efficient way to upregulate Dscam in hemocytes and that expression of extracellular Dscam is peaking 24 hours post LPS injection.

Place, publisher, year, edition, pages
2019. , p. 29
Keywords [en]
LPS, Dscam, hemocyte, crayfish
National Category
Cell Biology
Identifiers
URN: urn:nbn:se:uu:diva-385216OAI: oai:DiVA.org:uu-385216DiVA, id: diva2:1323452
Educational program
Master Programme in Biology
Supervisors
Examiners
Available from: 2019-06-14 Created: 2019-06-12 Last updated: 2019-06-14Bibliographically approved

Open Access in DiVA

The full text will be freely available from 2023-06-19 12:00
Available from 2023-06-19 12:00

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