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The Effects of Mast Cell Proteases on Human Lung Cells Population
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Biology Education Centre.
2019 (English)Independent thesis Advanced level (degree of Master (Two Years)), 30 credits / 45 HE creditsStudent thesis
Abstract [en]

Asthma is a devastating disease characterized by airway obstruction, inflammation, and bronchial hyperreactivity. Previous studies have implicated mast cells in the pathology of asthma, yet the role and function of mast cells in the early stage of human asthma development cannot be investigated in vivo for ethical reasons. In the present study, we investigated the mast cell proteases actions on primary cell populations, including primary human lung fibroblast (HLF) and primary human small airway epithelial cells (HSAEC). Mast cell chymase caused significant morphological effects on primary HLF by degrading extracellular matrix (ECM), such as fibronectin and collagen I, and activation of pro-MMP2. Chymase also regulated the cytokine synthesis and gene expression in primary HLF after 24 h treatment. Both tryptase and chymase stimulated primary HSAEC proliferation. These results indicate that mast cell proteases can have a profound impact during asthmatic airway responses.

Place, publisher, year, edition, pages
2019. , p. 39
Keywords [en]
asthma, primary cell, tryptase, chymase
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-385409OAI: oai:DiVA.org:uu-385409DiVA, id: diva2:1324131
Educational program
Master Programme in Biology
Supervisors
Examiners
Available from: 2019-06-13 Created: 2019-06-13 Last updated: 2019-06-13Bibliographically approved

Open Access in DiVA

The full text will be freely available from 2020-06-30 12:00
Available from 2020-06-30 12:00

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