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Verification of CE-marked method, GeneXpert, for detection of influenza A and respiratory syncytial virus
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Microbiology.
2019 (English)Independent thesis Basic level (professional degree), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

Background and Aim

 Acute respiratory tract infections are common illnesses and leading causes of mortality worldwide. Among the wide range of pathogens that can cause these infections, influenza A-, and respiratory syncytial virus are among the most prevalent. To improve patient care, rapid and accurate detection methods are critical. Cepheid GeneXpert Xpress Flu/RSV (Xpert) is a rapid and accurate detection instrument that amplifies and detects the genetic material of influenza A-, influenza B- and respiratory syncytial virus.

The purpose of this study was to establish Xpert performance on clinical samples in comparison with the Clinical Virology’s standard RT-PCR method.

Method and Result

In the study, 107 frozen nasopharynx samples were analyzed on Xpert. Xpert Influenza A method had a sensitivity of 97 % and specificity at 100 %. Xpert respiratory syncytial virus method had a similar sensitivity and specificity, both at 100 %.

Conclusion

Xpert Flu/RSV is easy to use, and no previous technical experience or knowledge is needed. Xpert has a short turn-around-time, which makes it well suited for point-of-care diagnostics. Furthermore, because the instrument has high sensitivity and specificity the test results are reliable.    

Place, publisher, year, edition, pages
2019. , p. 19
Keywords [en]
Respiratory Virus, Sample-to-answer, Clinical study, Rapid diagnostics, Point-of-care diagnostics
National Category
Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:uu:diva-385717OAI: oai:DiVA.org:uu-385717DiVA, id: diva2:1325518
Educational program
Biomedical Laboratory Science Programme; Biomedical Laboratory Science Programme
Supervisors
Examiners
Available from: 2019-08-21 Created: 2019-06-16 Last updated: 2019-08-21Bibliographically approved

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