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A strategy for the identification of protein architectures directly from ion mobility mass spectrometry data reveals stabilizing subunit interactions in light harvesting complexes
Karolinska Inst, Dept Microbiol Tumour & Cell Biol, Sci Life Lab, Tomtebodavagen 23A, SE-17165 Stockholm, Sweden.
Karolinska Inst, Dept Microbiol Tumour & Cell Biol, Sci Life Lab, Tomtebodavagen 23A, SE-17165 Stockholm, Sweden.ORCID iD: 0000-0002-2889-5200
Tallinn Univ, Sch Nat Sci & Hlth, Narva Mnt 25, EE-10120 Tallinn, Estonia.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Biochemistry.ORCID iD: 0000-0002-9804-5009
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2019 (English)In: Protein Science, ISSN 0961-8368, E-ISSN 1469-896X, Vol. 28, no 6, p. 1024-1030Article in journal (Refereed) Published
Abstract [en]

Biotechnological applications of protein complexes require detailed information about their structure and composition, which can be challenging to obtain for proteins from natural sources. Prominent examples are the ring-shaped phycoerythrin (PE) and phycocyanin (PC) complexes isolated from the light-harvesting antennae of red algae and cyanobacteria. Despite their widespread use as fluorescent probes in biotechnology and medicine, the structures and interactions of their noncrystallizable central subunits are largely unknown. Here, we employ ion mobility mass spectrometry to reveal varying stabilities of the PC and PE complexes and identify their closest architectural homologues among all protein assemblies in the Protein Data Bank (PDB). Our results suggest that the central subunits of PC and PE complexes, although absent from the crystal structures, may be crucial for their stability, and thus of unexpected importance for their biotechnological applications.

Place, publisher, year, edition, pages
WILEY , 2019. Vol. 28, no 6, p. 1024-1030
Keywords [en]
structural mass spectrometry, protein interactions, collision cross sections, protein complex stability, ion mobility, red algae
National Category
Biochemistry and Molecular Biology Structural Biology
Identifiers
URN: urn:nbn:se:uu:diva-386439DOI: 10.1002/pro.3609ISI: 000467751000006PubMedID: 30927297OAI: oai:DiVA.org:uu-386439DiVA, id: diva2:1330451
Funder
Swedish Research Council, 2013_08807 2015-00559Swedish Foundation for Strategic Research Available from: 2019-06-25 Created: 2019-06-25 Last updated: 2019-06-25Bibliographically approved

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Marklund, Erik

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