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Virus removal filtration of chemically defined Chinese Hamster Ovary cells medium with nanocellulose-based size exclusion filter
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Nanotechnology and Functional Materials.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Nanotechnology and Functional Materials.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Nanotechnology and Functional Materials.ORCID iD: 0000-0002-8105-2317
2019 (English)In: Biologicals (Print), ISSN 1045-1056, E-ISSN 1095-8320, Vol. 59, p. 62-67Article in journal (Refereed) Published
Abstract [en]

Sterility of bioreactors in biotherapeutic processing remains a significant challenge. Virus removal size-exclusion filtration is a robust and highly efficient approach to remove viruses. This article investigates the virus removal capacity of nanocellulose-based filter for upstream bioprocessing of chemically defined Chinese hamster ovary (CHO) cells medium containing Pluronic F-68 (PowerCHO (TM), Lonza) and supplemented with insulin-transferrinselenium (ITS) at varying process parameters. Virus retention was assessed by spiking ITS-supplemented PowerCHO (TM) medium with small-size Phi X174 phage (28 nm) as a surrogate for mammalian parvoviruses. The nanocellulose-based size exclusion filter showed high virus retention capacity (over 4 log(10)) and high flow rates (around 180 L m(-2) h(-1)). The filter had no impact on ITS supplements during filtration. It was further shown that the filtered PowerCHO (TM) medium supported cell culture growth with no impact on cell viability, morphology, and confluence. The results of this work show new opportunities in developing cost-efficient virus removal filters for upstream bioprocessing.

Place, publisher, year, edition, pages
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD , 2019. Vol. 59, p. 62-67
Keywords [en]
Mille-feuille filter paper, Upstream bioprocessing, Viral safety, Cell culture media, Steam sterilization
National Category
Nano Technology Nano Technology
Research subject
Engineering Science with specialization in Nanotechnology and Functional Materials
Identifiers
URN: urn:nbn:se:uu:diva-387972DOI: 10.1016/j.biologicals.2019.03.001ISI: 000469891500009PubMedID: 30871931OAI: oai:DiVA.org:uu-387972DiVA, id: diva2:1331653
Funder
Knut and Alice Wallenberg FoundationStiftelsen Olle Engkvist ByggmästareAvailable from: 2019-06-27 Created: 2019-06-27 Last updated: 2019-10-16

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Manukyan, LevonMihranyan, Albert

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