uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Detection of Leptospira in Urban Swedish Rats: Pest Control Interventions as a Promising Source of Rats Used for Surveillance
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology. Department of Microbiology, National Veterinary Institute (SVA), Uppsala, Sweden.ORCID iD: 0000-0002-0467-944X
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
Show others and affiliations
2019 (English)In: Vector Borne and Zoonotic Diseases, ISSN 1530-3667, E-ISSN 1557-7759, Vol. 19, no 6, p. 414-420Article in journal (Refereed) Published
Abstract [en]

Rat carcasses obtained from pest control interventions can potentially be used for an efficient surveillance of zoonotic diseases such as leptospirosis. To evaluate the performance of different laboratory methods for detection of pathogenic Leptospira spp., heart and kidney samples from wild Norway rats were analyzed by microscopic agglutination test (MAT, the gold standard), a commercial IgG enzyme-linked immunosorbent assay, and by an optimized quantitative PCR (secY qPCR, followed by sequencing). We found secY qPCR to be as sensitive as MAT for screening of Leptospira infection in pest control rats and selected secY qPCR for a larger screening of rats from urban and rural areas in central and southern Sweden. We identified secY qPCR positive rats from the cities Stockholm, Gothenburg, and Malmö, which were further confirmed by sequencing.

Place, publisher, year, edition, pages
2019. Vol. 19, no 6, p. 414-420
Keywords [en]
Norway rat, microscopic agglutination test, qPCR, zoonosis, Leptospira
National Category
Infectious Medicine
Identifiers
URN: urn:nbn:se:uu:diva-388728DOI: 10.1089/vbz.2017.2262ISI: 000474303200005PubMedID: 30785372OAI: oai:DiVA.org:uu-388728DiVA, id: diva2:1335061
Funder
Swedish Research Council, 2017-05807Available from: 2019-07-03 Created: 2019-07-03 Last updated: 2019-09-09Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMed

Authority records BETA

Strand, Tanja M.Lõhmus, MareJärhult, Josef D.Lundkvist, Åke

Search in DiVA

By author/editor
Strand, Tanja M.Lõhmus, MareJärhult, Josef D.Lundkvist, Åke
By organisation
Department of Medical Biochemistry and MicrobiologyInfection medicine
In the same journal
Vector Borne and Zoonotic Diseases
Infectious Medicine

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 40 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf