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Study of magnetic beads-DNA coils binding kinetics using a differential homogeneous magnetic assay
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Nanotechnology and Functional Materials. (Nanoteknologi och funktionella material, Nanotechnology and Functional Materials)
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2019 (English)In: Joint European Magnetic Symposia (JEMS) 2019 / [ed] The European Magnetism Association, Uppsala, 2019Conference paper, Oral presentation with published abstract (Refereed)
Abstract [en]

The binding kinetics of magnetic nanoparticles (MNPs) to rolling circle amplification products (RCPs) is investigated using a differential homogenous magnetic assay (DHMA)1. The DHMA utilizes a microfluidic device to measure the differential ac susceptibility signal between a reference and a test sample, taking advantage of the symmetry in a high-Tc SQUID gradiometer sensor2. The DHMA signal is related to the relative differences in the particle distribution of the two samples, and the background magnetic signal is thus eliminated. Therefore, minuscule changes in the nanoparticle’s concentration and size distribution of the test sample are directly detectable in the solution. This makes the DHMA a superior technique to characterize the binding interaction of the MNPs to biomolecules like RCPs specially at very low concentrations. The DHMA reveals that there is a competitive dynamic process between the MNP labelled RCPs and the unbound MNPs in the solution as a function of the RCP concentrations. The evidence of this dynamic in the signal fades as the MNP-RCP agglomerates are formed. The DHMA also shows that the smaller MNPs in the MNP size distribution take precedence over the larger MNP in immobilization on the RCPs. Comparing the DHMA responses with the turn-off detection method indicates that a full frequency range ac susceptibility observation is necessary when detecting low concentration of target RCPs. The findings are critical for understanding the underlying microscopic binding process and improving the assay performance. [1] Sepehri, S. et al, Differential homogeneous magnetic assay. Submitted. [2] Sepehri, S. et al. Volume-amplified magnetic bioassay integrated with microfluidic sample handling and high-Tc SQUID magnetic readout. APL Bioeng. 2, 016102 (2018).

Place, publisher, year, edition, pages
Uppsala, 2019.
National Category
Nano Technology
Research subject
Engineering Science with specialization in Nanotechnology and Functional Materials
Identifiers
URN: urn:nbn:se:uu:diva-389857OAI: oai:DiVA.org:uu-389857DiVA, id: diva2:1339569
Conference
Joint European Magnetic Symposia (JEMS), Uppsala, August 26-30 2019.
Available from: 2019-07-30 Created: 2019-07-30 Last updated: 2019-07-30

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Zardán Gómez de la Torre, Teresa

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