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A simple and cost-effective method for obtaining DNA from a wide range of animal wildlife samples
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Ecology and Genetics, Evolutionary Biology.ORCID iD: 0000-0002-0612-9230
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2017 (English)In: Conservation Genetics Resources, ISSN 1877-7252, E-ISSN 1877-7260, Vol. 9, no 4, p. 513-521Article in journal (Refereed) Published
Abstract [en]

Genetic analyses have become increasingly important in animal wildlife research where they have provided information of direct relevance to species and population management. An integral part of genetic analysis is the isolation of PCR-amplifiable DNA. However, addressing the issue of DNA isolation in animal wildlife research can be challenging due to the wide range of potential biological sources used to obtain DNA. Here, we present a modified silica-based DNA isolation method for obtaining PCR-amplifiable DNA from a wide range of biological sources typically encountered in animal wildlife research. We compared the present method with commonly used non-commercial and commercially available DNA extraction methods. Our results show that DNA yield, quality and purity (as assessed by PCR amplification) are qualitatively comparable, and in many cases even better, than commonly used non-commercial and commercially available DNA extraction methods. We describe the main factors that can affect DNA yield and provide variants of the basic protocol that can be performed according to the DNA source. The simplicity, versatility and cost-effectiveness of this method, as well as the absence of hazardous reagents, makes it a valuable addition to the molecular toolbox of any wildlife genetics laboratory.

Place, publisher, year, edition, pages
2017. Vol. 9, no 4, p. 513-521
Keywords [en]
DNA extraction, Non-invasive samples, Purification, Silica, Thiocyanate, Wildlife samples
National Category
Genetics
Identifiers
URN: urn:nbn:se:uu:diva-390735DOI: 10.1007/s12686-017-0735-zOAI: oai:DiVA.org:uu-390735DiVA, id: diva2:1342616
Available from: 2019-08-14 Created: 2019-08-14 Last updated: 2019-08-22Bibliographically approved

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Tusso, Sergio

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