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Droplet Dilution Unit Operation Including Bead Washing Using Integrated Acoustophoresis
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab. (EMBLA)ORCID iD: 0000-0001-7960-5878
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab. (EMBLA)ORCID iD: 0000-0001-7980-376x
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab. (EMBLA)ORCID iD: 0000-0002-1264-1337
2019 (English)Conference paper, Poster (with or without abstract) (Other academic)
Abstract [en]

This paper presents a microfluidic platform for on-chip droplet dilution where the bead recovery also can be controlled. The droplets containing 10 µm polystyrene beads can be diluted with high bead recovery. This platform involves 5 steps for on-chip dilution of the droplets: droplet generation, bead focusing, droplet splitting, pico-injection and serpentine mixing. Background signal in the droplets is significantly reduced with maintained bead recovery by this on-chip dilution method. The technology is applicable to many types of samples and does not require any labelling of the bioparticles.

Place, publisher, year, edition, pages
2019.
Keywords [en]
Droplet dilution, acoustophoresis, pico-injection, microfluidics, electrocoalescence
National Category
Engineering and Technology Embedded Systems
Research subject
Engineering Science with specialization in Microsystems Technology
Identifiers
URN: urn:nbn:se:uu:diva-390848OAI: oai:DiVA.org:uu-390848DiVA, id: diva2:1343005
Conference
The 20th International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers 2019 - EUROSENSORS XXXIII), 23-27 June 2019, Berlin, Germany
Available from: 2019-08-15 Created: 2019-08-15 Last updated: 2019-08-26Bibliographically approved

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Liu, ZhenhuaFornell, AnnaTenje, Maria

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