uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Gene miaA for post-transcriptional modification of tRNAXXA is important for morphological and metabolic differentiation in Streptomyces
Ivan Franko Natl Univ Lviv, Dept Genet & Biotechnol, 4 Hrushevskoho St, UA-79005 Lvov, Ukraine.
Ivan Franko Natl Univ Lviv, Dept Genet & Biotechnol, 4 Hrushevskoho St, UA-79005 Lvov, Ukraine.ORCID iD: 0000-0003-3296-8297
Ivan Franko Natl Univ Lviv, Dept Genet & Biotechnol, 4 Hrushevskoho St, UA-79005 Lvov, Ukraine.
Ivan Franko Natl Univ Lviv, Dept Phys Earth, 4 Hrushevskoho St, UA-79005 Lvov, Ukraine.
Show others and affiliations
2019 (English)In: Molecular Microbiology, ISSN 0950-382X, E-ISSN 1365-2958, Vol. 112, no 1, p. 249-265Article in journal (Refereed) Published
Abstract [en]

Members of actinobacterial genus Streptomyces possess a sophisticated life cycle and are the deepest source of bioactive secondary metabolites. Although morphogenesis and secondary metabolism are subject to transcriptional co-regulation, streptomycetes employ an additional mechanism to initiate the aforementioned processes. This mechanism is based on delayed translation of rare leucyl codon UUA by the only cognate tRNA(UAA)(Leu) (encoded by bldA). The bldA-based genetic switch is an extensively documented example of translational regulation in Streptomyces. Yet, after five decades since the discovery of bldA, factors that shape its function and peculiar conditionality remained elusive. Here we address the hypothesis that post-transcriptional tRNA modifications play a role in tRNA-based mechanisms of translational control in Streptomyces. Particularly, we studied two Streptomyces albus J1074 genes, XNR_1074 (miaA) and XNR_1078 (miaB), encoding tRNA (adenosine(37)-N6)-dimethylallyltransferase and tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase respectively. These enzymes produce, in a sequential manner, a hypermodified ms(2)i(6)A37 residue in most of the A36-A37-containing tRNAs. We show that miaB and especially miaA null mutant of S. albus possess altered morphogenesis and secondary metabolism. We provide genetic evidence that miaA deficiency impacts translational level of gene expression, most likely through impaired decoding of codons UXX and UUA in particular.

Place, publisher, year, edition, pages
2019. Vol. 112, no 1, p. 249-265
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-390986DOI: 10.1111/mmi.14266ISI: 000474705900016PubMedID: 31017319OAI: oai:DiVA.org:uu-390986DiVA, id: diva2:1343883
Funder
Swedish Research Council, 621-2016-03949Available from: 2019-08-19 Created: 2019-08-19 Last updated: 2019-08-19Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMed

Authority records BETA

Kirsebom, Leif

Search in DiVA

By author/editor
Yushchuk, OleksandrKirsebom, Leif
By organisation
Molecular Biology
In the same journal
Molecular Microbiology
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 27 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf