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An acoustofluidic platform for non-contact trapping of cell-laden hydrogel droplets compatible with optical microscopy
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab. (Embla)ORCID iD: 0000-0001-7980-376x
Lund University.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab. Natl Univ Singapore, Dept Biomed Engn, Fac Engn,. (Embla)ORCID iD: 0000-0001-9102-956x
Lund University.
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2019 (English)In: Biomicrofluidics, ISSN 1932-1058, E-ISSN 1932-1058, Vol. 13, article id 044101Article in journal (Refereed) Published
Abstract [en]

Production of cell-laden hydrogel droplets as miniaturized niches for 3D cell culture provides a new route for cell-based assays. Such production can be enabled by droplet microfluidics and here we present a droplet trapping system based on bulk acoustic waves for handling hydrogel droplets in a continuous flow format. The droplet trapping system consists of a glass capillary equipped with a small piezoelectric transducer. By applying ultrasound (4 MHz), a localized acoustic standing wave field is generated in the capillary, trapping the droplets in a well-defined cluster above the transducer area. The results show that the droplet cluster can be retained at flow rates of up to 76 mu l/min, corresponding to an average flow speed of 3.2 mm/s. The system allows for important operations such as continuous perfusion and/or addition of chemical reagents to the encapsulated cells with in situ optical access. This feature is demonstrated by performing on-chip staining of the cell nuclei. The key advantages of this trapping method are that it is label-free and gentle and thus well-suited for biological applications. Moreover, the droplets can easily be released on-demand, which facilitates downstream analysis. It is envisioned that the presented droplet trapping system will be a valuable tool for a wide range of multistep assays as well as long-term monitoring of cells encapsulated in gel-based droplets.

Place, publisher, year, edition, pages
2019. Vol. 13, article id 044101
National Category
Other Chemical Engineering
Research subject
Engineering Science with specialization in Microsystems Technology
Identifiers
URN: urn:nbn:se:uu:diva-392104DOI: 10.1063/1.5108583ISI: 000483884200007PubMedID: 31312286OAI: oai:DiVA.org:uu-392104DiVA, id: diva2:1346928
Funder
Swedish Research CouncilThe Crafoord FoundationStiftelsen Olle Engkvist ByggmästareSwedish Foundation for Strategic Research Available from: 2019-08-29 Created: 2019-08-29 Last updated: 2019-10-17Bibliographically approved

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Fornell, AnnaSearle, Sean S.Tenje, Maria

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