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The effects of different browning agents SuperHume, HuminFeed, and Reverse Osmosis Concentrate on the fitness and survival of Daphnia magna
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Ecology and Genetics, Limnology. Uppsala University, Disciplinary Domain of Science and Technology, Biology, Biology Education Centre.
2019 (English)Independent thesis Advanced level (degree of Master (Two Years)), 30 credits / 45 HE creditsStudent thesis
Abstract [en]

The browning of freshwater bodies due to increasing concentrations of dissolved organic carbon (DOC) is becoming more prevalent in light of the changing climate and is therefore a relevant topic of study within the scientific community. An obstacle to overcome however, is what to use as a source of carbon for scientific experiments. Reverse osmosis concentrate (ROC) is a natural source filtered from wetlands, though this can take a long time to collect. Other studies have turned to commercial products which are leonardite-based to simulate browning: HuminFeed, which comes as a powder or SuperHume, which is liquid. Here I present the results that test the effects of these browning agents on zooplankton. After a previous study indicated a severe impact on zooplankton in a lake mesocosm experiment, a controlled lab experiment was conducted. The model test organism Daphnia magna and copepods from a local lake were used to observe the effects of HuminFeed, SuperHume and ROC in a standard immobilisation and reproduction test using similar concentrations as the mesocosm experiment. The browning agents did not affect the immobilisation of D. magna or the copepods after 24 or 48 hours, however the reproduction test on D. magna resulted in a significant decrease in the total number of offspring produced in the HuminFeed treatment compared to the control. This study raises the question if the commercial product HuminFeed is an acceptable substance to use to mimic browning in lab or mesocosm experiments

Place, publisher, year, edition, pages
2019.
Keywords [en]
zooplankton, DOC, browning, life history traits
National Category
Ecology
Identifiers
URN: urn:nbn:se:uu:diva-392705OAI: oai:DiVA.org:uu-392705DiVA, id: diva2:1349303
Educational program
Master Programme in Biology
Supervisors
Examiners
Available from: 2019-09-24 Created: 2019-09-08 Last updated: 2019-09-24Bibliographically approved

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CiteExportLink to record
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