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Medical grade titanium on-chip: assessing the biological properties of biomaterials for bone regeneration
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.ORCID iD: 0000-0001-8055-5820
Uppsala University, Science for Life Laboratory, SciLifeLab. (Mikrosystemteknik, Microsystems Technology)
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology. Uppsala University, Science for Life Laboratory, SciLifeLab.ORCID iD: 0000-0002-1264-1337
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2019 (English)Conference paper, Poster (with or without abstract) (Other academic)
Abstract [en]

Medical grade titanium on-chip: assessing the biological properties of biomaterials for bone regeneration

 

Sarah-Sophia D. Carter1, Hugo Nguyen2, Milena Moreira1, Maria Tenje1, and Gemma Mestres1

1Department of Engineering Sciences, Science for Life Laboratory, Uppsala University, Sweden

2Department of Engineering Sciences, Uppsala University, Sweden

 

Introduction

Before entering the clinic, biomaterials need to be thoroughly evaluated, which requires accurate in vitro models. In this work, we have developed a microfluidic device that could be used to assess the biological properties of biomaterials, in a more in vivo-like environment than what is currently possible.

 

Methods

Our device consists of a polydimethylsiloxane (PDMS, Sylgard 184) microfluidic channel (l= 6 mm, w= 2 mm, h= 200 µm) and a titanium disc (Ti6Al4V, at bottom), held together by an additively manufactured fixture (Fig. 1A). PDMS was cured overnight at 65°C on a silicon wafer master. Once the microchannel and titanium disc were positioned, MC3T3-E1 pre-osteoblast-like cells were seeded (50,000 cells/cm2). After 5 hours incubation under standard culture conditions, flow was started (2 μl/min). As a control, MC3T3-E1 cells were seeded onto plain titanium discs off-chip. Cell viability and morphology were assessed after 20 hours by calcein-AM/propidium iodide (PI), staining live and dead cells respectively.

 

Results and discussion

Figure 1B and 1C show calcein-AM/PI stained MC3T3-E1 cells cultured on-chip and figure 1D shows the control, MC3T3-E1 cells cultured off-chip. The potential to culture cells in our chip was confirmed by the presence of a majority of viable cells (green) with a similar morphology as the control sample. The reason for the increased amount of dead cells (red) on-chip compared to the control needs to be further examined, which requires longer-term experiments.

Conclusion

We have set the first steps towards a microfluidic tool for the assessment of biological properties of biomaterials.

Place, publisher, year, edition, pages
2019.
Keywords [en]
Organ-on-chip, biomaterials, microfluidics
National Category
Engineering and Technology
Research subject
Engineering Science with specialization in Microsystems Technology
Identifiers
URN: urn:nbn:se:uu:diva-393277OAI: oai:DiVA.org:uu-393277DiVA, id: diva2:1352419
Conference
2nd European Organ-on-Chip Conference, EUROoC 2019, Graz, Austria, July 2-3, 2019
Funder
Knut and Alice Wallenberg Foundation, WAF 2016-0112Vattenfall AB, 2017-05051Available from: 2019-09-18 Created: 2019-09-18 Last updated: 2019-09-20Bibliographically approved

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Carter, Sarah-SophiaNguyen, HugoMoreira, MilenaTenje, MariaMestres, Gemma

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