uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Development and Validation of a CE Method for the Determination of Tetracyclines with Capacitively Coupled Contactless Conductivity Detection
KU Leuven Univ Leuven, Dept Pharmaceut & Pharmacol Sci, Pharmaceut Anal, O&N 2,Herestr 49,PB 923, B-3000 Leuven, Belgium.
KU Leuven Univ Leuven, Dept Pharmaceut & Pharmacol Sci, Pharmaceut Anal, O&N 2,Herestr 49,PB 923, B-3000 Leuven, Belgium.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Medicinal Chemistry, Analytical Pharmaceutical Chemistry. Kantisto BV, Callenburglaan 22, NL-3742 MV Baarn, Netherlands.ORCID iD: 0000-0001-8409-4443
KU Leuven Univ Leuven, Dept Pharmaceut & Pharmacol Sci, Pharmaceut Anal, O&N 2,Herestr 49,PB 923, B-3000 Leuven, Belgium.
Show others and affiliations
2019 (English)In: Chromatographia, ISSN 0009-5893, E-ISSN 1612-1112, Vol. 82, no 9, p. 1395-1403Article in journal (Refereed) Published
Abstract [en]

In this study, a simple and robust capillary electrophoresis method with capacitively coupled contactless conductivity detection ((CD)-D-4) is developed for the determination of the tetracycline antibiotics (1) tetracycline, (2) chlortetracycline and (3) oxytetracycline. An uncoated, fused silica capillary (60.2 cm long, 75 mu m i.d.) and a solution of 50 mM tris(hydroxymethyl) aminomethane, 50 mM l-histidine and 5 mM methyl-beta-cyclodextrin, without pH adjustment (pH 8.76), was used as background electrolyte. Electrophoresis at + 25 kV showed a rapid analysis with sufficient resolution among the three antibiotics in the order of tetracycline, chlortetracycline and oxytetracycline. Successive inter-injection rinsing (20 psi) of the capillary ensured intra- and inter-day repeatability (0.9-2.2% RSD and 2.0-4.5% RSD, respectively, for relative peak areas). The method showed satisfactory performance in terms of selectivity, accuracy (99.3-101.4%) and linearity (R-2 = 0.999). Finally, the method was applied to commercial samples of tetracycline, oxytetracycline and chlortetracycline. This method can be applied for rapid quality control in developing countries in particular, and across the globe in general.

Place, publisher, year, edition, pages
SPRINGER HEIDELBERG , 2019. Vol. 82, no 9, p. 1395-1403
Keywords [en]
Capillary electrophoresis, Capacitively coupled contactless conductivity detection, Tetracycline antibiotics, Validation
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:uu:diva-393720DOI: 10.1007/s10337-019-03755-4ISI: 000481888100010OAI: oai:DiVA.org:uu-393720DiVA, id: diva2:1356154
Available from: 2019-10-01 Created: 2019-10-01 Last updated: 2019-10-18Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full text

Authority records BETA

Sänger - van de Griend, Cari

Search in DiVA

By author/editor
Sänger - van de Griend, Cari
By organisation
Analytical Pharmaceutical Chemistry
In the same journal
Chromatographia
Analytical Chemistry

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 35 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf