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Development of a method using paperpoint stick sampling to determine Ti concentration in saliva with ICP-MS
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry.
Division of Prosthetic Dentistry, Department of Odontology, Umeå University.
(English)Manuscript (preprint) (Other academic)
Abstract [en]

Peri-implantitis is a disease that causes an inflammatory process in soft tissues as well as bone loss around dental implants integrated into bone. The relationship between peri-implantitis and titanium presence has previously been established and also a correlation between the inflammation severity and Ti concentration in biopsies from patients with dental implants. Biopsy can be considered as an invasive sampling procedure and therefore there is an interest to study alternative sampling methods, such as collecting saliva with paperpoint sticks. The small volume absorbed by the paperpoint sticks requires a sensitive detection method for Ti, therefore ICP-MS was used. To minimize the effect of common polyatomic interferences on Ti, the use of He as a collision gas and complexation using NH3 as reaction gas were investigated and both methods were compared to each other. Three sample preparation techniques were investigated: leaching, ultrasonic extraction and microwave bomb digestion. The use of paperpoint sticks for sampling was shown to be a valid approach with simple leaching in combination with ICP-MS using either He- or NH3-gas. The use of NH3-gas to create a 131Ti-complex, required more technical expertise, but gave a 2 times lower detection limit compared to using He as a collision gas.

National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:uu:diva-394935OAI: oai:DiVA.org:uu-394935DiVA, id: diva2:1360051
Available from: 2019-10-10 Created: 2019-10-10 Last updated: 2019-10-10
In thesis
1. Method development for the analysis of complex samples with inductively coupled plasma mass spectrometry
Open this publication in new window or tab >>Method development for the analysis of complex samples with inductively coupled plasma mass spectrometry
2019 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

In this thesis the development of methods for handling the problems associated with analyzing trace elements in complex matrixes using inductively coupled plasma mass spectrometry is presented. Trace elements such as Cu, Fe, Se, and Zn, to name a few, do play important roles in different organisms. Therefore it can be of importance to study trace elements in different samples of biological origin. As trace elements are low in abundance, sensitive instrumental techniques such as inductively coupled plasma mass spectrometry (ICP-MS) are required for accurate determination. Due to the complexity of samples with biological origin, careful method development, both regarding the sample preparation and instrumental analysis has to be performed to minimize negative effects on the instrument signal and introduction of interferences.

For example the metal contents of mink livers were analyzed, after bomb digestion to investigate if the metal concentration could be linked to changes in the organ morphology as well as the minks’ environment. Morphological changes and capture locations could be linked to the metals investigated. The investigation of the elemental composition of cerebrospinal fluid from chronic pain patients using spinal cord stimulation electrode treatment on the other hand required less harsh sample treatment. No correlation between the spinal cord stimulation and element concentration could be found, but differences between patients and the control group were presented hinting that chronic pain intrinsically could affect the cerebrospinal fluid metal concentration. Another bodily fluid of interest is saliva and the use of paperpoint sticks as a sampling technique for Ti in saliva was investigated. As Ti is interfered by several components expected to be found in saliva, the use of reaction or collision gas was also investigated to reduce the effects of interferences. Simple leaching of the paperpoint sticks together with complexing the Ti with NH3 as reaction gas was shown to be optimal. Finally, how the selection of internal standard would be affected by the use of reaction and collision gases was also investigated. With collision gas most internal standards worked fine, while for reaction gas internal standard selection was harder. For elements with high ionization energy such as As, Se and Zn the choice of internal standard was very dependent on matrix. While ICP-MS suffers from problems when analyzing samples with complex matrixes many of them can be minimized by proper method development as shown in this thesis.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2019. p. 52
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 1865
Keywords
ICP-MS, trace elements, complex matrix.
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:uu:diva-394936 (URN)978-91-513-0772-5 (ISBN)
Public defence
2019-11-29, B41, BMC, Husargatan 3, Uppsala, 13:15 (English)
Opponent
Supervisors
Available from: 2019-11-08 Created: 2019-10-10 Last updated: 2019-11-08

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