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Homogeneous Differential Magnetic Assay
Chalmers Univ Technol, Dept Microtechnol & Nanosci MC2, SE-41296 Gothenburg, Sweden.ORCID iD: 0000-0002-2145-1340
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Nanotechnology and Functional Materials.
Chalmers Univ Technol, Dept Microtechnol & Nanosci MC2, SE-41296 Gothenburg, Sweden;Univ Gothenburg, MedTech West, SE-40530 Gothenburg, Sweden;Univ Gothenburg, Inst Neurosci & Physiol, SE-40530 Gothenburg, Sweden.
RISE Res Inst Sweden, SE-41133 Gothenburg, Sweden.
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2019 (English)In: ACS SENSORS, ISSN 2379-3694, Vol. 4, no 9, p. 2381-2388Article in journal (Refereed) Published
Abstract [en]

Assays are widely used for detection of various targets, including pathogens, drugs, and toxins. Homogeneous assays are promising for the realization of point-of-care diagnostics as they do not require separation, immobilization, or washing steps. For low concentrations of target molecules, the speed and sensitivity of homogeneous assays have hitherto been limited by slow binding kinetics, time-consuming amplification steps, and the presence of a high background signal. Here, we present a homogeneous differential magnetic assay that utilizes a differential magnetic readout that eliminates previous limitations of homogeneous assays. The assay uses a gradiometer sensor configuration combined with precise microfluidic sample handling. This enables simultaneous differential measurement sample containing a synthesized Vibrio cholerae target and a negative control sample, which reduces the background signal and increases the readout speed. Very low concentrations of targets down to femtomolar levels are thus detectable without any additional amplification of the number of targets. Our homogeneous differential magnetic assay method opens new possibilities for rapid and highly sensitive diagnostics at the point of care.

Place, publisher, year, edition, pages
AMER CHEMICAL SOC , 2019. Vol. 4, no 9, p. 2381-2388
Keywords [en]
homogeneous differential magnetic assay, magnetic nanoparticle, volumetric detection, rolling circle amplification, Brownian relaxation, binding kinetics
National Category
Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:uu:diva-395849DOI: 10.1021/acssensors.9b00969ISI: 000488424100021PubMedID: 31397152OAI: oai:DiVA.org:uu-395849DiVA, id: diva2:1365483
Funder
Swedish Foundation for Strategic Research , SBE13-0125Swedish Research Council, 2015-03640Knut and Alice Wallenberg FoundationAvailable from: 2019-10-25 Created: 2019-10-25 Last updated: 2019-10-25Bibliographically approved

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Zardán Gómez de la Torre, TeresaStrömme, Maria

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