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Nanoarrays on Passivated Aluminum Surface for Site-Specific Immobilization of Biomolecules
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences. Uppsala University. (Shiyu Li)ORCID iD: 0000-0003-4948-8353
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology.ORCID iD: 0000-0002-5226-1427
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Electrical Engineering, Solid-State Electronics.ORCID iD: 0000-0003-4317-9701
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2018 (English)In: ACS Applied Bio Materials, ISSN 2576-6422, Vol. 1, no 1, p. 125-135Article in journal (Refereed) Published
Abstract [en]

The rapid development of biosensing platforms for highly sensitive and specific detection raises the desire of precise localization of biomolecules onto various material surfaces. Aluminum has been strategically employed in the biosensor system due to its compatibility with CMOS technology and its optical and electrical properties such as prominent propagation of surface plasmons. Herein, we present an adaptable method for preparation of carbon nanoarrays on aluminum surface passivated with poly(vinylphosphonic acid) (PVPA). The carbon nanoarrays were defined by means of electron beam induced deposition (EBID) and they were employed to realize site-specific immobilization of target biomolecules. To demonstrate the concept, selective streptavidin/neutravidin immobilization on the carbon nanoarrays was achieved through protein physisorption with a significantly high contrast of the carbon domains over the surrounding PVPA-modified aluminum surface. By adjusting the fabrication parameters, local protein densities could be varied on similarly sized nanodomains in a parallel process. Moreover, localization of single 40 nm biotinylated beads was achieved by loading them on the neutravidin-decorated nanoarrays. As a further demonstration, DNA polymerase with a streptavidin tag was bound to the biotin-beads that were immobilized on the nanoarrays and in situ rolling circle amplification (RCA) was subsequently performed. The observation of organized DNA arrays synthesized by RCA verified the nanoscale localization of the enzyme with retained biological activity. Hence, the presented approach could provide a flexible and universal avenue to precise localizing various biomolecules on aluminum surface for potential biosensor and bioelectronic applications. 

Place, publisher, year, edition, pages
2018. Vol. 1, no 1, p. 125-135
National Category
Other Electrical Engineering, Electronic Engineering, Information Engineering
Identifiers
URN: urn:nbn:se:uu:diva-397410DOI: 10.1021/acsabm.8b00037OAI: oai:DiVA.org:uu-397410DiVA, id: diva2:1371504
Available from: 2019-11-20 Created: 2019-11-20 Last updated: 2020-01-15Bibliographically approved

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Li, ShiyuZeng, ShuangshuangChen, LeiZhang, ZhenHjort, Klas

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Department of Engineering SciencesDepartment of Immunology, Genetics and PathologySolid-State ElectronicsMicrosystems Technology
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