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Evidence of Intra-individual SSU Polymorphisms in Dark-spored Myxomycetes (Amoebozoa)
CSIC, Real Jardin Bot, Plaza Murillo 2, Madrid 28014, Spain.
Uppsala University, Music and Museums, Museum of Evolution.
CSIC, Real Jardin Bot, Plaza Murillo 2, Madrid 28014, Spain.
2019 (English)In: Protist, ISSN 1434-4610, E-ISSN 1618-0941, Vol. 170, no 5, article id 125681Article in journal (Refereed) Published
Abstract [en]

The nuclear small subunit rRNA gene (SSU or 18S) is a marker frequently used in phylogenetic and barcoding studies in Amoebozoa, including Myxomycetes. Despite its common usage and the confirmed existence of divergent copies of ribosomal genes in other protists, the potential presence of intra-individual SSU variability in Myxomycetes has never been studied before. Here we investigated the pattern of nucleotide polymorphism in the 5' end fragment of SSU by cloning and sequencing a total of 238 variants from eight specimens, each representing a species of the dark-spored orders Stemonitidales and Physarales. After excluding singletons, a relatively low SSU intra-individual variability was found but our data indicate that this might be a widely distributed phenomenon in Myxomycetes as all samples analyzed possessed various ribotypes. To determine if the occurrence of multiple SSU variants within a single specimen has a negative effect on the circumscription of species boundaries, we conducted phylogenetic analyses that revealed that clone variation may be detrimental for inferring phylogenetic relationships among some of the specimens analyzed. Despite that intra-individual variability should be assessed in additional taxa, our results indicate that special care should be taken for species identification when working with closely related species.

Place, publisher, year, edition, pages
ELSEVIER GMBH , 2019. Vol. 170, no 5, article id 125681
Keywords [en]
Amoebozoa, intra-individual variability, Myxomycetes, small subunit rRNA gene
National Category
Biological Systematics
Identifiers
URN: urn:nbn:se:uu:diva-398437DOI: 10.1016/j.protis.2019.125681ISI: 000497783400003PubMedID: 31586669OAI: oai:DiVA.org:uu-398437DiVA, id: diva2:1375774
Available from: 2019-12-06 Created: 2019-12-06 Last updated: 2019-12-06Bibliographically approved

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Zamora, Juan Carlos

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