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Mechanism and kinetics of lipid bilayer formation in solid-state nanopores
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Solid State Electronics. Uppsala University.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Solid State Electronics.ORCID iD: 0000-0003-4948-8353
Linköping University.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Solid State Electronics.ORCID iD: 0000-0003-4395-7905
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2019 (English)In: Langmuir, ISSN 0743-7463, E-ISSN 1520-5827Article in journal (Refereed) Submitted
Abstract [en]

Solid-state nanopores provide a highly versatile platform for rapid electrical detection and analysis of single molecules. Lipid bilayer coating of the nanopores can reduce non-specific analyte adsorption to the nanopore sidewalls and increase the sensing selectivity by providing possibilities for tethering specific ligands in a cell-membrane mimicking environment. However, mechanism and kinetics of lipid bilayer formation from vesicles remain unclear in the presence of nanopores. In this work, we used a silicon-based, truncated-pyramidal nanopore array as the support for lipid bilayer formation. Lipid bilayer formation in the nanopores was monitored in real-time by the change in ionic current through the nanopores. Statistical analysis revealed that a lipid bilayer is formed from instantaneous rupture of individual vesicle upon adsorption in the nanopores, differing from the generally agreed mechanism that lipid bilayers form at a high vesicle surface coverage on a planar support. The2dependence of the lipid-bilayer formation process as a function of applied bias, vesicle size and concentration was systematically studied. In addition, the non-fouling properties of the lipid bilayer coated nanopores were demonstrated during long single stranded DNA translocation through the nanopore array. The findings indicate that lipid bilayer formation process can be modulated by introducing nanocavities intentionally on the planar surface to create active sites or changing the vesicle size and concentration.

Place, publisher, year, edition, pages
2019.
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Engineering and Technology
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URN: urn:nbn:se:uu:diva-399725OAI: oai:DiVA.org:uu-399725DiVA, id: diva2:1379057
Available from: 2019-12-16 Created: 2019-12-16 Last updated: 2020-01-08
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Zeng, ShuangshuangLi, ShiyuWen, ChenyuZhang, Shi-LiZhang, Zhen

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