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A Spatiotemporal Organ-Wide Gene Expression and Cell Atlas of the Developing Human Heart
KTH Royal Inst Technol, Dept Gene Technol, Sci Life Lab, Stockholm, Sweden.
KTH Royal Inst Technol, Dept Gene Technol, Sci Life Lab, Stockholm, Sweden;Stockholm Univ, Dept Biochem & Biophys, Sci Life Lab, Natl Bioinformat Infrastruct Sweden, Stockholm, Sweden.
KTH Royal Inst Technol, Dept Gene Technol, Sci Life Lab, Stockholm, Sweden.
Stockholm Univ, Dept Biochem & Biophys, Sci Life Lab, Stockholm, Sweden.
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2019 (English)In: Cell, ISSN 0092-8674, E-ISSN 1097-4172, Vol. 179, no 7, p. 1647-+Article in journal (Refereed) Published
Abstract [en]

The process of cardiac morphogenesis in humans is incompletely understood. Its full characterization requires a deep exploration of the organ-wide orchestration of gene expression with a single-cell spatial resolution. Here, we present a molecular approach that reveals the comprehensive transcriptional landscape of cell types populating the embryonic heart at three developmental stages and that maps cell-type-specific gene expression to specific anatomical domains. Spatial transcriptomics identified unique gene profiles that correspond to distinct anatomical regions in each developmental stage. Human embryonic cardiac cell types identified by single-cell RNA sequencing confirmed and enriched the spatial annotation of embryonic cardiac gene expression. In situ sequencing was then used to refine these results and create a spatial subcellular map for the three developmental phases. Finally, we generated a publicly available web resource of the human developing heart to facilitate future studies on human cardiogenesis.

Place, publisher, year, edition, pages
CELL PRESS , 2019. Vol. 179, no 7, p. 1647-+
National Category
Cell and Molecular Biology Developmental Biology
Identifiers
URN: urn:nbn:se:uu:diva-401182DOI: 10.1016/j.cell.2019.11.025ISI: 000502546200020PubMedID: 31835037OAI: oai:DiVA.org:uu-401182DiVA, id: diva2:1383081
Funder
Knut and Alice Wallenberg FoundationSwedish Research CouncilSwedish Research Council Formas, 2017-01066_3Available from: 2020-01-07 Created: 2020-01-07 Last updated: 2020-01-07Bibliographically approved

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Reimegård, Johan

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