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Cell Type-Specific Expression of Testis Elevated Genes Based on Transcriptomics and Antibody-Based Proteomics
Univ Rennes, INSERM, UMR S 1085, EHESP,Irset Inst Rech Sante Environm & Travail, F-35042 Rennes, France;Univ Rennes, Protim, F-35042 Rennes, France.ORCID iD: 0000-0002-7461-5433
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Clinical and experimental pathology.
KTH Royal Inst Technol, Sch Engn Sci Chem Biotechnol & Hlth, Sci Life Lab, S-17121 Stockholm, Sweden.ORCID iD: 0000-0002-3721-8586
KTH Royal Inst Technol, Sch Engn Sci Chem Biotechnol & Hlth, Sci Life Lab, S-17121 Stockholm, Sweden.
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2019 (English)In: Journal of Proteome Research, ISSN 1535-3893, E-ISSN 1535-3907, Vol. 18, no 12, p. 4215-4230Article in journal (Refereed) Published
Abstract [en]

One of the most complex organs in the human body is the testis, where spermatogenesis takes place. This physiological process involves thousands of genes and proteins that are activated and repressed, making testis the organ with the highest number of tissue-specific genes. However, the function of a large proportion of the corresponding proteins remains unknown and testis harbors many missing proteins (MPs), defined as products of protein-coding genes that lack experimental mass spectrometry evidence. Here, an integrated omits approach was used for exploring the cell type-specific protein expression of genes with an elevated expression in testis. By combining genome-wide transcriptomics analysis with immunohistochemistry, more than 500 proteins with distinct testicular protein expression patterns were identified, and these were selected for in-depth characterization of their in situ expression in eight different testicular cell types. The cell type-specific protein expression patterns allowed us to identify six distinct clusters of expression at different stages of spermatogenesis. The analysis highlighted numerous poorly characterized proteins in each of these clusters whose expression overlapped with that of known proteins involved in spermatogenesis, including 85 proteins with an unknown function and 60 proteins that previously have been classified as MPs. Furthermore, we were able to characterize the in situ distribution of several proteins that previously lacked spatial information and cell type specific expression within the testis. The testis elevated expression levels both at the RNA and protein levels suggest that these proteins are related to testis-specific functions. In summary, the study demonstrates the power of combining genome-wide transcriptomics analysis with antibody-based protein profiling to explore the cell type-specific expression of both well-known proteins and MPs. The analyzed proteins constitute important targets for further testis-specific research in male reproductive disorders.

Place, publisher, year, edition, pages
AMER CHEMICAL SOC , 2019. Vol. 18, no 12, p. 4215-4230
Keywords [en]
testis, reproduction, spermatogenesis, antibody-based proteomics, missing proteins, protein evidence, immunohistochemistry, transcriptomics
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-400735DOI: 10.1021/acs.jproteome.9b00351ISI: 000502164100015PubMedID: 31429579OAI: oai:DiVA.org:uu-400735DiVA, id: diva2:1386451
Funder
Knut and Alice Wallenberg FoundationAvailable from: 2020-01-17 Created: 2020-01-17 Last updated: 2020-01-17Bibliographically approved

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Hikmet, FeriaLindskog, Cecilia

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