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Improvement of photobiological hydrogen production by suspended and immobilized cells of the N-2-fixing cyanobacterium Fischerella muscicola TISTR 8215
Chulalongkorn Univ, Dept Biochem, Lab Cyanobacterial Biotechnol, Fac Sci, Bangkok 10330, Thailand;Chulalongkorn Univ, Program Biotechnol, Fac Sci, Bangkok 10330, Thailand.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Molecular Biomimetics. (Mikrobiell kemi)ORCID iD: 0000-0001-7256-0275
Chulalongkorn Univ, Dept Biochem, Lab Cyanobacterial Biotechnol, Fac Sci, Bangkok 10330, Thailand;Royal Soc Thailand, Acad Sci, Bangkok 10300, Thailand.
2019 (English)In: Journal of Applied Phycology, ISSN 0921-8971, E-ISSN 1573-5176, Vol. 31, no 6, p. 3527-3536Article in journal (Refereed) Published
Abstract [en]

To develop H-2 photoproduction by using the N-2-fixing cyanobacterium Fischerella muscicola TISTR 8215, a novel strain isolated from soil in Thailand, the factors affecting H-2 production were investigated in this study. Enhanced H-2 production in suspension culture was obtained when adapting the cells under N-2-fixing condition (modified AA medium) with continuous illumination of 250 mu mol photons m(-2) s(-1) under aerobic condition for 24 h, followed by further incubation under anaerobic condition for 9 h for production phase. The maximum H-2 production rate was 38.5 mu mol mg(-1) chl a h(-1). Low concentration of Fe2+ and Mo6+, essential elements for nitrogenase, enhanced H-2 production. The enhanced H-2 production was accompanied by the upregulation of nifD. On the other hand, an increased hupL transcript level was observed when there was a decrease of H-2 production. In cells immobilization, 1.5% (w/v) agar-immobilized cells had a 23-fold increase in maximum H-2 yield compared with that using free cell suspension at the same cell concentration, i.e., 7.48 mmol H-2 L-1 by immobilized cells and 0.32 mmol H-2 L-1 by suspended cells. Moreover, cell immobilization in agar could prolong H-2 production up to 108 h. This study underlines the strategies toward enhanced and sustained H-2 production from cyanobacteria. Furthermore, it will pave the way for large-scale production of biohydrogen to be used as an eco-friendly energy resource.

Place, publisher, year, edition, pages
SPRINGER , 2019. Vol. 31, no 6, p. 3527-3536
Keywords [en]
Cyanobacteria, H-2 production, Cell immobilization, Fischerella
National Category
Microbiology
Identifiers
URN: urn:nbn:se:uu:diva-406198DOI: 10.1007/s10811-019-01881-yISI: 000507642200016OAI: oai:DiVA.org:uu-406198DiVA, id: diva2:1412392
Available from: 2020-03-06 Created: 2020-03-06 Last updated: 2020-03-06Bibliographically approved

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Lindblad, Peter

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