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Biological Laboratory X-Ray Microscopy
KTH Royal Inst Technol, AlbaNova Univ Ctr, Dept Appl Phys, Biomed & Xray Phys, S-10691 Stockholm, Sweden.
KTH Royal Inst Technol, AlbaNova Univ Ctr, Dept Appl Phys, Biomed & Xray Phys, S-10691 Stockholm, Sweden.
Karolinska Inst, Dept Microbiol Tumor & Cell Biol, S-17177 Solna, Sweden.
KTH Royal Inst Technol, AlbaNova Univ Ctr, Dept Appl Phys, Biophys, S-10691 Stockholm, Sweden.
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2019 (English)In: X-RAY NANOIMAGING: INSTRUMENTS AND METHODS IV / [ed] Lai, B Somogyi, A, SPIE-INT SOC OPTICAL ENGINEERING , 2019, article id 111120TConference paper, Published paper (Refereed)
Abstract [en]

Zone-plate-based soft x-ray microscopes operating in the water window allow high-resolution and high-contrast imaging of intact cells in their near-native state. Laboratory-source-based x-ray microscopes are an important complement to the accelerator-based instruments, providing high accessibility and allowing close integration with other cell-biological techniques. Here we present recent biological applications using the Stockholm laboratory water-window x-ray microscope, which is based on a liquid-nitrogen-jet laser-plasma source. Technical improvements to the microscope in the last few years have resulted in increased x-ray flux at the sample and significantly improved stability and reliability. In addition to this, vibrations in key components have been measured, analyzed and reduced to improve the resolution to 25 nm half-period. The biological applications include monitoring the development of carbon-dense vesicles in starving human embryonic kidney cells (HEK293T), imaging the interaction between natural killer (NK) cells and HEK293T target cells, and most recently studying a newly discovered giant DNA virus and the process of viral replication inside a host amoeba. All biological imaging was done on cryo-frozen hydrated samples in 2D and in some cases 3D.

Place, publisher, year, edition, pages
SPIE-INT SOC OPTICAL ENGINEERING , 2019. article id 111120T
Series
Proceedings of SPIE, ISSN 0277-786X, E-ISSN 1996-756X ; 11112
Keywords [en]
X-ray microscopy, water-window, laboratory, resolution, cell imaging, cryo
National Category
Atom and Molecular Physics and Optics
Identifiers
URN: urn:nbn:se:uu:diva-407113DOI: 10.1117/12.2531165ISI: 000511110600017ISBN: 978-1-5106-2918-9 (electronic)OAI: oai:DiVA.org:uu-407113DiVA, id: diva2:1415714
Conference
Conference on X-Ray Nanoimaging - Instruments and Methods IV held at SPIE Optical Engineering + Applications Conference, AUG 11-12, 2019, San Diego, CA
Funder
Swedish Research CouncilAvailable from: 2020-03-19 Created: 2020-03-19 Last updated: 2020-03-19Bibliographically approved

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Reddy, Hemanth K.N.Svenda, Martin

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