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A PDMS-based disposable microfluidic sensor for CD4+ lymphotic counting
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Oncology, Radiology and Clinical Immunology.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Analytical Chemistry.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Microsystems Technology.
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2008 (English)In: Biomedical microdevices (Print), ISSN 1387-2176, E-ISSN 1572-8781, Vol. 10, no 6, 851-857 p.Article in journal (Refereed) Published
Abstract [en]

A refined sensor for CD4+ lymphocyte count was developed and evaluated by comparison to flow cytometry. The micropillar structured sensor surface was cast in PDMS polymer and surface modified to gain biocompatibility and CD4-cell capturing properties. The sensor works by pure capillary action and sample filling and rinsing is performed without external equipment. Whole blood samples showed acceptable agreement (79%) with flow cytometry, however when diluting the blood in PBS buffer we discovered that a larger number of cells were drawn into the sensor microchannel compared to the initial sample, explained by enhanced shear-induced cell migration. Using plasma or PBS with glycerol or albumin additives as diluting media greatly influenced this cell behavior, showing the importance of controlling the dilution media when working with devices based on capillary filling. The sensors need to be further tested with blood samples with lower CD4-counts (<500 cells/μl), which are clinically relevant.

Place, publisher, year, edition, pages
2008. Vol. 10, no 6, 851-857 p.
Keyword [en]
CD4 count, Point-of-care, Capillary wetting, HIV monitoring, Dilution media
National Category
Analytical Chemistry Engineering and Technology
Research subject
Analytical Chemistry
Identifiers
URN: urn:nbn:se:uu:diva-88540DOI: 10.1007/s10544-008-9199-yISI: 000259246400009PubMedID: 18546075OAI: oai:DiVA.org:uu-88540DiVA: diva2:158557
Available from: 2009-02-03 Created: 2009-02-03 Last updated: 2016-04-13Bibliographically approved

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Bergquist, JonasNikolajeff, Fredrik

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Microsystems TechnologyDepartment of Oncology, Radiology and Clinical ImmunologyAnalytical Chemistry
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Biomedical microdevices (Print)
Analytical ChemistryEngineering and Technology

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