uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Post-antifungal effect of amphotericin B and voriconazole against germinated Aspergillus fumigatus conidia
Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences, Infectious Diseases.
2008 (English)In: Journal of Antimicrobial Chemotherapy, ISSN 0305-7453, Vol. 61, no 6, 1309-11 p.Article in journal (Refereed) Published
Abstract [en]

OBJECTIVES: The post-antifungal effect (PAFE) of amphotericin B and voriconazole on germinated Aspergillus fumigatus conidia was studied using the BacT/Alert detection system based on fungal CO(2) production. METHODS: Germinated conidia of A. fumigatus were exposed to 1-10x MIC of amphotericin B for 1 and 4 h and to 2.5-40x MIC of voriconazole for 4 and 24 h. After removal of the drug by washing, similar numbers of exposed and control germlings were inoculated into Pedi-BacT culture bottles. CO(2) production was automatically monitored until the bottles signalled positive. The difference in time for positive signals in drug-exposed and control bottles was used to calculate the PAFE. RESULTS: The killing rate of amphotericin B against germlings was both concentration- and time-dependent, as has been previously found for actively growing hyphae. Similarly, voriconazole showed fungicidal effect after 24 h of exposure, but not after 4 h. Amphotericin B induced a long concentration- and time-dependent PAFE, whereas voriconazole resulted in a short and dose-independent PAFE that was significantly longer after 24 h than after 4 h of exposure. CONCLUSIONS: An automated method is presented for the determination of PAFE on filamentous fungi using quantifiable numbers of germinated conidia. In contrast to previous results obtained from conidia, this method could demonstrate a PAFE of amphotericin B on Aspergillus that shared characteristics similar to that on Candida spp.

Place, publisher, year, edition, pages
2008. Vol. 61, no 6, 1309-11 p.
Keyword [en]
azoles, filamentous fungi, polyenes
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-88553DOI: 10.1093/jac/dkn129ISI: 000256172900020PubMedID: 18367461OAI: oai:DiVA.org:uu-88553DiVA: diva2:158566
Available from: 2009-02-19 Created: 2009-02-03 Last updated: 2009-03-20Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed
By organisation
Infectious Diseases
In the same journal
Journal of Antimicrobial Chemotherapy
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle ScholarTotal: 1 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 160 hits
ReferencesLink to record
Permanent link

Direct link