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A simple method for long-term storage of Acanthamoeba species
Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences, Infectious Diseases.
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2009 (English)In: Parasitology Reseach, ISSN 0932-0113, Vol. 104, no 4, 935-7 p.Article in journal (Refereed) Published
Abstract [en]

We present a novel and simple technique for storing live Acanthamoeba for long periods of time. The amoebae are maintained at refrigerator temperatures in a peptone-yeast extract-glucose (PYG) medium normally used for cultivation. Using this method, we obtained survival rates of at least 4 years for Acanthamoeba polyphaga and 3 years for Acanthamoeba castellanii and Acanthamoeba rhysodes. Advantages of this storage method are: (1) it is quick and simple, (2) inexpensive, (3) does not require encystment before storage, (4) resuscitation of cysts can be achieved within a week of culture in PYG medium at 27 degrees C, and does not require co-culture with bacteria or any special equipment.

Place, publisher, year, edition, pages
2009. Vol. 104, no 4, 935-7 p.
National Category
Infectious Medicine
URN: urn:nbn:se:uu:diva-88716DOI: 10.1007/s00436-008-1304-xISI: 000263500600026PubMedID: 19089450OAI: oai:DiVA.org:uu-88716DiVA: diva2:158934
Available from: 2009-02-26 Created: 2009-02-05 Last updated: 2009-03-20Bibliographically approved

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