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Adenosine 5'-carboxylic acid peptidyl derivatives as inhibitors of protein kinases
Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
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1999 In: Bioorg. Med. Chem. Lett., Vol. 9, 1447-52 p.Article in journal (Refereed) Published
Place, publisher, year, edition, pages
1999. Vol. 9, 1447-52 p.
URN: urn:nbn:se:uu:diva-89445OAI: oai:DiVA.org:uu-89445DiVA: diva2:160883
Available from: 2001-10-03 Created: 2001-10-03Bibliographically approved
In thesis
1. Studies on the Differential Specificity of Protein Kinases and Its Applications
Open this publication in new window or tab >>Studies on the Differential Specificity of Protein Kinases and Its Applications
2001 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Protein kinases are enzymes that catalyse the phosphoryl transfer from the g-phosphate of ATP to acceptor amino acids in proteins. The specificity of selected model protein kinases was studied at three different levels using a) novel bi-substrate-analogue inhibitors, b) synthetic peptide substrates and c) mutated protein substrate analogues.

A new class of protein kinase bi-substrate-analogue inhibitors was designed on the basis of adenosine-5’-carboxylic acid derivatives, where a short arginine containing peptide was attached to the 5'-carbon atom of the adenosine sugar moiety via a linker chain. These compounds showed high inhibitory potential against two basophilic protein kinases, the protein kinase A (PKA) and protein kinase C (PKC), with IC50 values in the nanomolar range, but no inhibitory activity towards the acidophilic kinases CK1 and CK2. The inhibitors were efficiently applied for affinity purification of PKA using MgATP as well as L-arginine as eluting agents.

Ca2+-dependent protein kinase (CDPK-1) was purified from maize seedlings and its substrate specificity was studied using a set of synthetic peptides. These were derived from the phosphorylatable sequence RVLSRLHS(15)VRER of maize sucrose synthase 2 (SuSy2), and a consensus sequence motif A/LXRXXSXRZR (where X denotes a position with no strict amino acid requirements and Z a position strictly not tolerating arginine) was defined from a study using arrays of systematically varied peptides attached to cellulose membrane (SPOTsTM membranes). The SuSy2 derived peptides were also found to be efficient substrates for mammalian PKC, but showed low reactivity in the case of PKA. On the basis of this peptide motif, a positionally oriented peptide library approach based on ESI-MS detection of phosphopeptides in initial velocity conditions was designed for quantitative kinetic characterization of protein kinase specificity profiles. On the basis of the obtained data an optimal peptide substrate for PKC, FRRRRSFRRR, was designed.

The specificity of protein kinase A was studied using site-directed mutagenesis in the phosphorylation site of L-type pyruvate kinase (L-PK), and comparison of the obtained data with the data from previous studies on structurally altered peptide substrates revealed that amino acid alterations in short peptide substrates cause stronger effects on the phosphorylation rate than the corresponding alterations in the protein substrate L-PK.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2001. 48 p.
Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, ISSN 0282-7476 ; 1073
Biochemistry, Protein kinases, protein kinase inhibitors, substrate specificity, peptide libraries, affinity chromatography, Biokemi
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Research subject
urn:nbn:se:uu:diva-1389 (URN)91-554-5116-0 (ISBN)
Public defence
2001-10-04, lecture hall C10:305, Biomedical Center (BMC), Uppsala, 09:15
Available from: 2001-10-03 Created: 2001-10-03Bibliographically approved

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