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Regulation of the release of eosinophil cationic protein by eosinophil adhesion
Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Medical Sciences.
2000 In: Clin. Exp. Allergy, Vol. 30, 794-806 p.Article in journal (Refereed) Published
Place, publisher, year, edition, pages
2000. Vol. 30, 794-806 p.
Identifiers
URN: urn:nbn:se:uu:diva-89483OAI: oai:DiVA.org:uu-89483DiVA: diva2:160969
Available from: 2001-10-15 Created: 2001-10-15Bibliographically approved
In thesis
1. Granulocyte Adhesion to Matrix Proteins and the Effect on the Release of Granule Proteins: Development of a Simple Method and its Application in Experimental and Clinical Studies
Open this publication in new window or tab >>Granulocyte Adhesion to Matrix Proteins and the Effect on the Release of Granule Proteins: Development of a Simple Method and its Application in Experimental and Clinical Studies
2001 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Granulocyte adhesion and release of their granule proteins are key steps during selective accumulation of a certain cell to an inflammatory site. Eosinophils are specifically recruited to sites of allergic inflammation and parasitic infection, whereas neutrophil influx predominates in bacterial infection and rheumatoid arthritis.

A simple, reliable and convenient method was developed for the measurement of granulocyte adhesion and release of granule proteins by using the normal population of granulocytes. The design allows simultaneous quantitative assessment of eosinophil and neutrophil adhesion to proteins and degranulation.

Using this method, manganese ions (Mn2+) induced a higher level of eosinophil adhesion to fibronectin, fibrinogen and albumin as compared with neutrophils. PMA induced comparable levels of eosinophil and neutrophil adhesion. F-MLP stimulated a rapid, short-term adhesion of neutrophils to fibrinogen.

In the same conditions PMA alone stimulated a dose-dependent release of ECP from cells that adhered to both fibronectin and fibrinogen. Meanwhile, Mn2+ amplified the release of ECP induced by PMA. Furthermore, release of ECP was shown to be associated with cell death.

PMA, in combination with Mn2+, induced a marked release of ~ 80%of the intracellular content of lactoferrin and HNL in neutrophils. PMA or f-MLP alone induced 30-40% release of lactoferrin and HNL. A maximal release of MPO of 15-20% was obtained from neutrophils stimulated by PMA and Mn2+. Release of lactoferrin and HNL showed a significant negative relationship to the viability of cells.

Stimulated by PMA, eosinophils from pollen-atopic patients during early pollen season displayed a markedly enhanced adhesion and release of ECP of eosinophils compared with eosinophils from the references. Priming with IL-5 caused a significantly higher adhesion and release of ECP by eosinophils in response to PMA. GM-CSF priming enhanced eosinophil adhesion in response to PAF and PMA plus Mn2+, but did not enhance the release of ECP.

In conclusion, the assay allows a simple quantification of eosinophil and neutrophil adhesion, as well as degranulation by using the normal population of granulocytes. Cellular adhesion plays an important role in the regulation of both eosinophil and neutrophil degranulation, but adhesion and degranulation can be induced separately.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2001. 46 p.
Series
Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, ISSN 0282-7476 ; 1071
Keyword
Medical sciences, Eosinophils, Neutrophils, Adhesion, Release of ECP, Extracellular matrix proteins, Granulocytes, MEDICIN OCH VÅRD
National Category
Medical and Health Sciences
Research subject
Clinical Chemistry
Identifiers
urn:nbn:se:uu:diva-1437 (URN)91-554-5112-8 (ISBN)
Public defence
2001-10-22, Lecture hall Robergsalen, University Hospital, entrance 40, Uppsala, 13:15
Opponent
Available from: 2001-10-15 Created: 2001-10-15Bibliographically approved

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